Natural infection of Hippeastrum hybridum with Capsicum chlorosis virus and Iris yellow spot virus in Iran

Document Type : Short Article

Authors

1 Ornamental plants research center/Manager

2 Ornamental Plants Research center- Mahallat, Iran

3 Citrus and Subtropical Fruits Research center, Ramsar, Iran

Abstract

Hippeastrum/amaryllis (Hippeastrum hybridum Hort.) belongs to Amaryllidaceae. Plants of the hippeastrum exhibiting virus-like symptoms of chlorotic ring spots and concentric oval patterns were found in the collection of ornamental geophytes of the Ornamental Plants Research Center, Mahallat, Iran. Amplification of the genome using general primers of the Orthotospovirus genus, and specific primers of capsicum chlorosis virus (CaCV) led to the amplification of the expected fragments in some of the collected samples. Sequencing of amplified fragments proved the infection of some of the samples with CaCV and iris yellow spot virus (IYSV). No simultaneous infection with these two viruses was observed in any of the samples. This is the first report of the occurrence of members of the genus Orthotospovirus on the hippeastrum plant from Iran.

Keywords

References

Alexandre M., Duarte L., Rivas E., Cilli A., Harakava R., Galleti S. and Kitajima E. 2011. Hippeastrum mosaic virus diagnosed in Hippeastrum and Eucharis in Brazil. Journal of Plant Pathology 93 (3) :643-649.
Azimi M.H., Alavijeh, M.K. 2020. Morphological traits and genetic parameters of Hippeastrumhybridum. Ornamental Horticulture 26: 579-590.
Basavaraj Y., Siwach J., Kumar A., Bhattarai A., Qayoom N., Baranwal V.K. and Jain R.K. 2020. First report of natural infection by Capsicum chlorosis virus on Amaryllis (Hippeastrum hybridum) plants from India. Plant Disease 104:3086-3086.
Bayat H., Hassani-Mehraban A., Safaie N. and Shams-bakhsh M. 2018. Molecular and biological characterization of an isolate of capsicum chlorosis virus from Iran. Journal of Plant Pathology 100:163-170.
Bayat H., Hassani-Mehraban A., Safaie N. and Shams-bakhsh M. 2022. Nucleotide sequence and genetic analysis of the small RNAs of two Iranian isolates of Iris yellow spot virus (IYSV) from leek and onion. Iranian Journal of Plant Pathology 58(1):19-34.
Beikzadeh N., Bayat H., Jafarpour B., Rohani H., Peters D. and Hassani‐Mehraban A. 2012. Infection of Alstroemeria Plants with Tomato yellow ring virus in Iran. Journal of Phytopathology 160:45-47.
Berniak H. 2016. Characterization of a new Tomato spotted wilt virus isolates found in Hippeastrum hybridum (Hort.) plants in Poland. Journal of Horticultural Research 24:5-12.
Chen C.C., Huang C.H., Cheng Y.H., Chen T.C., Yeh S.D. and Chang C.A. 2009. First Report of Capsicum chlorosis virus Infecting Amaryllis and Blood Lily in Taiwan. Plant Disease 93:1346-1346.
ChenT.C., Li J.T., Lin Y.P., Yeh Y.C., KangY.C., Huang L.H. and Yeh S.D. 2012a. Genomic characterization of Calla lily chlorotic spot virus and design of broad-spectrum primers for detection of tospoviruses. Plant Pathology 61: 183-194
Chen S., Tai C., Wang H. 2012b. Characterization of Hippeastrum latent virus, HiLV. Plant Pathology Bulletin 21:259-268.
Dong J., Yin Y., Fang Q., McBeath J. and Zhang Z. 2013. A new tospoviruscausing chlorotic ringspot on Hippeastrum sp. in China. Virus genes 46:567-570.
Ghotbi T., Shahraeen N. and Winter S. 2005. Occurrence of tospoviruses in ornamental and weed species in Markazi and Tehran provinces in Iran. Plant Disease 89(4): 425-429.
Golnaraghi A.R., Pourrahim R., Farzadfar S., Ohshima K., Shahraeen N. and Ahoonmanesh A. 2007. Incidence and distribution of Tomato yellow fruit ring virus on soybean in Iran. Plant Pathology Journal (Faisalabad) 6:14-21.
Hasani-Mehraban A., Westenberg M., Verhoeven J., van de Vossenberg B., Kormelink R. and Roenhorst J. 2016. Generic RT-PCR tests for detection and identification of tospoviruses. Journal of Virological Methods 233:89-96.
Huang K.S., Li S.L., Sun J.H., Wang Y.C., Jan F.J. and Chen T.C. 2018. Development of a generic method for inspection of tospoviruses. European Journal of Plant Pathology 150:457-469.
Karthikeyan G., Nagendran K., Kumari S., Priyanka R., Senthilraja C. and Haokip B.D. 2021. Tospovirus Diseases of Ornamental Plants: Characterization, Identification, Diagnosis and Management. In: S.K. Raj, R.K. Gaur and Z. Yin (eds). Virus Diseases of Ornamental Plants.Springer, Singapore pp 1-21.
Kritzman A., Lampel M., Raccah B. and Gera A. 2001. Distribution and transmission of Iris yellow spot virus. Plant Disease 85:838-842.
Kumar S., Stecher G., Li M., Knyaz C.and Tamura K.2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35:1547.
Kunkalikar S.R., Sudarsana P., Rajagopalan P., Zehr U.B. and Ravi K.S. 2010. Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India. Archives of Virology 155:1047-1057.
Malandraki I., Driessen A., Varveri C. and Vassilakos N. 2016. First Report of Hippeastrummosaic virus in Hippeastrum sp. in Greece. Plant Disease 100:869.
Masoomi-Aladizgeh F., Jabbari L., Khayam Nekouei R.and Aalami A.2016. A Simple and Rapid System for DNA and RNA Isolation from Diverse Plants Using Handmade Kit. Protocol Exchange. https://doi.org/10.21203/rs.2.1347/v2
Meerow A.W., Broschat T.K. and Kane M.E. 1990. Breeding of new hippeastrum cultivars using diploid species: the F1-evaluation. HortScience 25(9): 1142e-1142.
Pappu H., Jones R. and Jain R. 2009. Global status of tospovirus epidemics in diverse cropping systems: Successes achieved and challenges ahead. Virus Research 141:219-236.
Raj S., Snehi S., Kumar S. and Khan M. 2009. First molecular detection and identification of a potyvirus associated with severe mosaic disease of amaryllis (Hippeastrum hybridum Hort.) in India. Australasian Plant Disease Notes 4:50-53.
Sharma A. and Kulshrestha S. 2014. First report of Amaranthus sp. as a natural host of capsicum chlorosis virus in India. Virus Disease 25:412-413.
Sharman M., Thomas J., Tree D. and Persley D. 2020.  Natural host range and thrips transmission of capsicum chlorosis virus in Australia. Australasian Plant Pathology 49:45-51.
Xu X., Tang W., Gao R., Yang S., Li F., Sun H. and Lu X. 2017. First Report of Hippeastrum mosaic virus in Hippeastrum spp. in Mainland China. Plant Disease 101:1064-1064.
Zheng Y.X., Chen C.C. and Jan F.J. 2011. Complete nucleotide sequence of capsicum chlorosis virus isolated from Phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses. Archives of Virology 156:421-432.
Iranian Journal of Plant Pathology
Volume 59, Issue 1
May 2024
Pages 49-55

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