EXPRESSIONS OF DEFENSE GENES AGAINST Ralstonia solanacearum IN SUSCEPTIBLE AND RESISTANT POTATO GENOTYPES UNDER IN Vitro CONDITIONS

MOSLEMKHANI, K.; MOZAFARI, J.; SHAMS BAKHSH, M.; MOHAMADI GOLTAPE, E.

EXPRESSIONS OF DEFENSE GENES AGAINST Ralstonia solanacearum IN SUSCEPTIBLE AND RESISTANT POTATO GENOTYPES UNDER IN Vitro CONDITIONS

Document Type : Research Article

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Abstract

Bacterial wilt of potato, a sever and devastating disease caused by Ralstonia solanacearum occurs in tropical and subtropical area. Using resistant cultivars is important in control of this disease. Discovery of plant resistant mechanisms and induced plant responses against pathogen may provide valuable information for improved resistant cultivars. In this direction, the expression pattern of some important defense genes against R. solanacearum in potato commercial cultivars like, Marfona (susceptible), Els (tolerant) of Solanum tuberosum and resistant genotype of Solanum phureja was evaluated over times after inoculation The level of expression of evaluated defense genes including Chitinase A, Chitinase B, Glucanase, PR-10a in the resistant genotype of S. phureja was higher than the other two commercial cultivars. Results show expression of Chitinase A and PR-10a in S. phureja and tolerant cultivar, Els have increase compared to susceptible cultivar Marfona. Expression of defense protein and antimicrobial compounds were also evaluated based on the growth rate of the fungus Fusarium solani on culture media containing extract of induced plants. The growth rate of the fungus decreased on the culture containing extracts from the resistant genotype, compared that of susceptible cultivar.

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