Molecular characterization of glycoprotein and phosphoprotein genes of a BYSMV isolate from Iran and transmission efficiency of different isolates

ALMASI, R.; AFSHARIFAR, A.; NIAZI, A.; IZADPANAH, K.

Molecular characterization of glycoprotein and phosphoprotein genes of a BYSMV isolate from Iran and transmission efficiency of different isolates

Document Type : Research Article

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Abstract

Barley yellow striate mosaic virus (BYSMV) belongs to the genus Cytorhabdovirusin the family Rhabdoviridae. It causes mosaic and chlorotic striation in gramineous plants and is transmitted by Laodelphax striatellus in a propagative manner. In the present study, nucleotide sequences ofglycoprotein and phosphoprotein genes of BYSMV Zanjan isolate are reported. Following thepurification of viral nucleocapsid, the nucleotide sequence of G and P genes was determined using random-PCR method (rPCR) followed by PCR with specific primers. Putative amino acid sequences of the two proteins were aligned with other members of Rhabdoviridae using CLUSTAL W software and the phylogenetic trees were depicted. BYSMV G and P proteins showed the highest similarity to the Northern cereal mosaic virus (NCMV). In phylogenetic analysis, the viruses were grouped into two main clusters reflecting the Cytorhabdovirus and Nucleorhabdovirus genera. These results support our previous phylogenetic analysis based on polymerase gene.Analysis of transmission efficiency showed that Zanjan, Naghadeh and Sero isolates were transmitted with a higher efficiency than Mashhad isolate by the same biotype of L. striatellus.

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