Allele analysis of microsatellite loci in Rhizoctonia solani AG-1 IA populations from paddy fields of three locations in north of Iran*

PADASHT-DEHKAEI, F.; ZALA, M.; JAVAN-NIKKHAH, M.; CERESINI, P.; OKHOVVAT, S. M.; MACDONALD, B.

Allele analysis of microsatellite loci in Rhizoctonia solani AG-1 IA populations from paddy fields of three locations in north of Iran*

Document Type : Research Article

Authors

Abstract

Six populations of Rhizoctonia solani AG-1 IA were sampled from three naturally infested rice fields in Rasht, Tonekabon and Amol counties, in north of Iran. Allele fragment analyses were performed using fluorescent-labeled primers from nine microsatellite (SSR) loci. The ranges of allele and genotype frequencies were from 0.002 to 0.962 and from 0.40 to10.32 percent respectively among 252 isolates. The frequency of 13, 11 and 9 alleles was zero on five, six and three loci across geographical populations of Rasht, Amol and Tonekabon respectively, so from them two loci weren't in Hardy–Weinberg Equilibrium (HWE) in each population.While the allele 145 bp at a locus TC12 was fixed in the Amol populations at a frequency of 1.0. As there was populations didn't show an even distribution of allele and genotype frequencies. This situation represents the gradual fixation and removal of some alleles in populations of the pathogen in the paddies of northern Iran.

Keywords