STUDY ON PATHOTYPES DIVERSITY AND VIRULENCE FACTORS OF Puccinia triticina ERIKSSON, THE CAUSAL AGENT OF WHEAT BROWN RUST IN IRAN *

NIAZMAND, A. R.; AFSHARI, F.; ABBASI, M.; REZAEE, S.

STUDY ON PATHOTYPES DIVERSITY AND VIRULENCE FACTORS OF Puccinia triticina ERIKSSON, THE CAUSAL AGENT OF WHEAT BROWN RUST IN IRAN *

Document Type : Research Article

Authors

Abstract

Leaf rust of wheat caused byPuccinia triticina Eriksson occurs annually in different parts of Iran. Samples of Puccinia triticinawere collected from rust-infected wheat leaves from different regions of Iran during 2007-2008 cropping seasons. Fifty one isolates were collected from different parts of Iran. Purification, inoculation and multiplication of isolates were conducted at the seedling stage of susceptible Bolani, under green house conditions. After inoculation on the seedlings of brown rust of wheat near isogenic lines, a total of fifty one pathotype were identified based on Avirulenve/Virulence formula. In two years some collected isolates showed similar virulence phenotypes included: TKTT, PKTT, PJTT, PHRR, PKRT, PHRT, PGTT and TCTT and the others were unique. Marivan I and Avaz II isolates showed the most virulence and Gorgan II was the least virulence in 2007. In 2008, Borojerd I and Ardebil I showed the more aggressive and Ahvaz II was the least. Among all the collected isolates during two years no virulence were detected on plants with Lr9, Lr19, Lr25 and Lr28resistance genes. Eight and six clusters were identified based on drawn denderogram at 0.76 cutting line in 2007 and 2008, respectively.

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