The Iranian isolate of tomato leaf curl virus (TLCV-Ir; GenBank Acc. No. AY297924) is a whitefly (Bemisia tabaci) transmitted begomovirus causing leaf curl disease of tomato in Iranshahr (Sistan and Baloochestan province, Iran). To test the infectivity of a full-length 2763 nucleotide DNA of this virus, a partial dimer (head-to-tail) was constructed in the binary vector pBin19 and transferred into Agrobacterium tumefaciens strain C58. A range of potential hosts including Lycopersicon esculentum,Nicotiana tabacum, N. benthamiana, Datura stramonium and some other indicator plants were agroinoculated with a bacterial culture of this construct. Mild curling and yellowing symptoms were observed on newly developed leaves of tomato plants within 30 to 45 days post-inoculation (dpi). However, the next phase of symptom development on tomato occurred 2-3 months after inoculation and produced elongated spindly shoots, smaller leaves and severe leaf cupping on the newly developed leaves. N. benthamiana plants agroinoculated with TLCV-Ir alsoshowed severe symptoms including leaf curling and leaf cupping within 21 to 30 dpi. No symptoms were observed on other agroinoculated plants but dot blot and PCR analysis using TLCV-Ir-specific probes and primers, respectively, confirmed the presence of viral DNA in Datura stramonium and N. tabacum plants indicating that these plants are symptomless hosts of the TLCV-Ir. On the basis of host range, symptom severity, timing of symptom appearance and the level of replication of the virus in host plants in comparison with a severe isolate of TLCV, i.e., the Australian isolate of TLCV, TLCV-Ir is considered as a mild isolate. Transmission of TLCV-Ir from agro-infected tomato plants to healthy tomato seedling was achieved with one adult of B. tabaci and the inoculated plants showed typical symptoms of the disease. The infectious clone of TLCV-Ir can be used as a source of this virus in various studies including screening of resistance cultivars.
BEHJATNIA, S. A. A., EINI GANDOMANI, O., & RASOULPOUR, R. (2009). Infectivity of the cloned genome, transmission and host range of an Iranian isolate of tomato leaf curl geminivirus. Iranian Journal of Plant Pathology, 45(1), 54-69.
MLA
S. A. A. BEHJATNIA; OMID EINI GANDOMANI; RASOUL RASOULPOUR. "Infectivity of the cloned genome, transmission and host range of an Iranian isolate of tomato leaf curl geminivirus". Iranian Journal of Plant Pathology, 45, 1, 2009, 54-69.
HARVARD
BEHJATNIA, S. A. A., EINI GANDOMANI, O., RASOULPOUR, R. (2009). 'Infectivity of the cloned genome, transmission and host range of an Iranian isolate of tomato leaf curl geminivirus', Iranian Journal of Plant Pathology, 45(1), pp. 54-69.
VANCOUVER
BEHJATNIA, S. A. A., EINI GANDOMANI, O., RASOULPOUR, R. Infectivity of the cloned genome, transmission and host range of an Iranian isolate of tomato leaf curl geminivirus. Iranian Journal of Plant Pathology, 2009; 45(1): 54-69.
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