CONSTRUCTION AND FUNCTIONAL ANALYSIS OF A PATHOGEN INDUCIBLE SYNTHETIC PROMOTER IN RESPONSE  TO SOME BIOTIC AND ABIOTIC STRESSES IN CANOLA

SHOKOUHIFAR, F.; ZAMANI, M. R.; MOTALLEBI, M.; MOSAVI, A.; MALBOBI, M. A.

CONSTRUCTION AND FUNCTIONAL ANALYSIS OF A PATHOGEN INDUCIBLE SYNTHETIC PROMOTER IN RESPONSE TO SOME BIOTIC AND ABIOTIC STRESSES IN CANOLA

Document Type : Research Article

Authors

Abstract

The synthetic promoters can be very beneficial in order to decrease the complexity of natural promoters and are effective to improve the plant breeding programs. In this study two synthetic promoters SynP1 and MinP were constructed and fused with β-glucuronidase as reporter gene. Functional analysis showed SynP1 promoter significantly responds to salicylic acid, chitin and cell wall released elicitors of Rhizoctonia solani and Fusarium graminearum. Methyl jasmonate, wounding and environmental conditions (cold, heat and UV) did not induce the SynP1 promoter. The background expression of SynP1 promoter was approximately the same as minimal promoter (MinP) and wild type plants. These results showed that SynP1 promoter is related to salicylic acid defense signaling pathway.

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