Apple Powdery Mildew Resistance Genes in Iranian Wild Apple Genotypes

Document Type : Research Article

Authors

1 Isfahan University of Technology

2 MSc. graduated/ Isfahan University of Technology

3 Post Doc. Researcher/ Isfahan University of Technology

4 Department of Plant Protection, College of Agriculture, Yasouj University, Yasouj, Iran.

Abstract

Apple (Malus domestica Borkh) is one of the most valuable agricultural products in the Rosaceae family because of its high nutritional and economic value. Powdery mildew of apple which is caused by Podosphaera leucotricha, is one of the primary fungal diseases with an extensively negative impact on the quality and quantity of apple production. In recent years, the use of resistant cultivars for controlling powdery mildew of apple has been considered. For this purpose, the genes Pl1, Pld, Plbj, and RGA (Resistance Gene Analogs) candidate resistance genes were detected in 47 genotypes of wild apples collected from West Azerbaijan, Golestan, Fars, Chaharmahal va Bakhtiari and Isfahan Provinces. The presence of NBS-LRR conserved domains and P-loop NTPase motif in NBS-LRR-like protein, which encode by the RGA in N-terminal, were confirmed in wild apple genotypes of Iran. Pl1 resistance gene was detected in all collected samples in this research except one. Pld gene just was observed in three out of the 47 samples, and the Plbj resistance gene was detected in just 13 samples of all wild genotypes collected from cold areas of Azerbaijan and Isfahan provinces; the sequences of this gene was introduced to the Global database. Detection of resistance genes and RGAs could help in developing resistant apple varieties in future.

Keywords


  1. AltschulS. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W. and Lipman D. J.1997.  Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs. Nucleic Acids Research 25:3389-3402.
  2. Amirchakhmaghi N., Yousefzadeh H., Hosseinpour B., Espahbodi K., Aldaghi M. and Cornille A. 2018. First insight into genetic diversity and population structure of the Caucasian wild apple (Malus orientalis Uglitzk.) in the Hyrcanian forest (Iran) and its resistance to apple scab and powdery mildew. Genetic Resources and Crop Evolution 65:1255–1268.
  3. Baek D. E. and Choi C. 2013. Identification of resistance gene analogs in Korean wild apple germplasm collections. Genetics and Molecular Research 12: 483-493.
  4. Caffier V. and Parisi L. 2007. Development of apple powdery mildew on sources of resistance to Podosphaera leucotricha, exposed to an inoculum virulent against the major resistance gene Pl-2. Plant Breeding126:319–322.
  5. Caffier V. and Laurens F. 2005. Breakdown of Pl2, a major gene of resistance to apple powdery mildew, in a French experimental orchard. Plant Pathology 54:116–124.
  6. Dunemann F. and Schuster M. 2009. Genetic characterization and mapping of the major powdery mildew resistance gene Plbj from Malus baccata jackii. Acta Horticulturae 814:791-798.
  7. Dunemann F., Peil A., Urbanietz A. and Garcia–Libreros T. 2007. Mapping of the apple powdery mildew resistance gene Pl1 and its genetic association with an NBS-LRR candidate resistance gene. Plant Breeding 126:476-481.
  8. Ershad D. 2009. Fungi of Iran. Iranian Research Institute of Plant Protection, 539Pp.
  9. Gelvonauskis B. and Gelvonauskiene D. 2003. Inheritance of resistance to powdery mildew and apple blotch in progenies of scab-resistant apple cultivars. Biologija 1:73-76.
  10. Hall T.  A. 1999. “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT”. Nucleic Acids Symposium Series 41:95-98.
  11. James C. M and Evans KM. 2004. Identification of molecular markers linked to the mildew resistance genes Pld and Plw in apple. Acta Horticulturae 663: 141-146.
  12. James C.M., Clarke J.B. and Evans K. M. 2004. Identification of molecular markers linked to the mildew resistance gene pld in apple. Theoretical and Applied Genetics 106:178–183
  13. Katoh K. and Standley D. M. 2013. MAFFT Multiple Sequence Alignment Software Version 7: Improvements in performance and usability. Molecular Biology and Evolution 30:772–780.
  14. Kellerhals M., Baumgartner I. O., Leumann L., Frey J.E. and Patocchi A. 2013. Progress in Pyramiding Disease Resistances in Apple Breeding. Acta Horticulturae 976, 487-491.
  15. Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120.
  16. Korban S. S. and Riemer S. E. 1990. Genetics and histology of powdery mildew resistance in apple. Euphytica 48:261-267.
  17. Maric S., Lukic M., Cerovic R., Mitrovic M. and Boškovic R. 2010. Application of molecular markers in apple breeding. Genetika2:359-375.
  18. Marine S. C., Yoder K. S. and Baudoin A. 2010. Powdery mildew of apple. The Plant Health Instructor. Virginia Polytechnic Institute and State University, USA.
  19. Markussen T., Kruger J., Schmidt H. and Dunemann F. 1995. Identification of PCR-based markers linked to the powdery-mildew-resistance gene Pli from Malus robusta in cultivated apple. Plant Breeding 114:530-534.
  20. Mozaffarian V. 2005. Trees and shrubs of Iran. Farhang Moaser Publishers, Tehran, pp 1–1054.
  21. Meyers B. C., Kaushik S. and Nandety R. S. 2005. Evolving disease resistance genes. Current Opinion in Plant Biology 8:129–134.
  22. Murray M. G. and Thompson W. F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research8: 4321-4326.
  23. Nei M. 1973. Analysis of Gene Diversity in Subdivided Populations. Proceeding of the National Academy of Sciences of the United States of America 70:3321-332.
  24. Patzak J., Paprstein F.and Henychova A. 2011. Identification of Apple Scab and Powdery Mildew Resistance Genes in Czech Apple (Malus × domestica) Genetic Resources by PCR Molecular Markers. Czech Journal of Genetics and Plant Breeding 47:156–165
  25. Perazzolli M., Malacarne G., Baldo A., Righetti L., Bailey A., Fontana P., Velasco R., Malnoy M. 2014. Characterization of Resistance Gene Analogues (RGAs) in Apple (Malus x domestica Borkh.) and their evolutionary history of the Rosaceae family. Plos One. 9(2):e83844.
  26. Sambrook J. and Russel D. W. 2001. Molecular cloning: A laboratory manual. Third edition. Cold Spring Harbour Laboratory Press. Cold Spring Harbour. NY.
  27. Sekhwal M. K., Li P, Lam I., Wang X., Cloutier S., You F. M. 2015. Disease Resistance Gene Analogs (RGAs) in Plants. International Journal of Molecular Sciences16:19248-19290.
  28. Sestras A. F., Pamfil D., Dan C., Bolboaca S. D., Jäntschi L., Sestras R. E. 2011. Possibilities to improve apple scab (venturia inaequalis (Cke.) Wint.) and powdery mildew (Podosphaera leucotricha (Ell. et Everh.) Salm.) resistance on apple by increasing genetic diversity using potentials of wild species. Australian Journal of Crop Science 5: 748–755.
  29. Tamura K., Dudley J., Nei M. and Kumar S. 2007. Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution 24:1596–1599.
  30. Wurms K.V. and Chee A. 2011. Control of powdery mildew (Podosphaera leucotricha) on apple seedlings using anhydrous milk fat and soybean oil emulsions. New Zealand Plant Protection 64:201-208.
  31. Xu X. M. 1999. Modeling and forecasting epidemics of apple powdery mildew (Podosphaera leucotricha). Plant Pathology 48:462-471.