Reaction of some Persian lime genotypes to ‘Candidatus Phytoplasma aurantifolia’ under greenhouse condition

Document Type : Research Article

Authors

1 Plant Protection Department, Faculty of Agriculture, university of Tabriz

2 Department of Plant Protection, Faculty of Agriculture, University of Tabriz

3 Citrus institute, Ramsar

4 Hormozghan

Abstract

Lime witches' broom disease associated with ‘Candidatus Phytoplasma aurantifolia’ is one of the most serious threats to lime production in Oman, United Arab Emirates and Iranian southern provinces. In this study, under warm condition (35-37°C and 25-27°C day/night) the reaction of four genotypes of Persian lime including TLCR10, TLCRO, TLC876 (tolerant genotypes) and RA (sensitive genotype) to ‘Ca. P. aurantifolia’ was investigated via graft inoculation. Also, under cool condition (24-26°C and 18- 20°C day/night) using the same inoculation method, the reaction of RA genotype to this pathogen was evaluated. Quantitative PCR was used for detection and quantification of ‘Ca. P. aurantifolia’ in the inoculated plants. At 48 weeks post-inoculation, the copy numbers of ‘Ca. P. aurantifolia’ per 100 ng of total plant DNA in TLCRO, TLC876 and TLCR genotypes was very low (17, 10 and 12, respectively) and no disease symptoms were observed. In RA genotype under warm condition, the mean copy numbers of ‘Ca. P. aurantifolia’ per 100 ng of plant total DNA was higher than that of cool condition and showed significant differences (P < 0.01) . Furthermore, the disease symptoms were only appeared under the warm condition. The results of the present study showed that TLC10, TLC876 and TLCRO genotypes had high tolerance to ‘Ca. P. aurantifolia’ and can be introduced as tolerant or resistant cultivars to ‘Ca. P. aurantifolia’. These tolerant genotypes can be used in genetic breeding programs to produce resistant and tolerant cultivars. The results also indicated that higher temperature may change susceptibility of

Keywords

References

Al-Yahyai R., Khan I., Al-Said F., Al-Sadi A., Al-Wahaibi A. and Deadman M. 2010. Status of Citrus aurantifolia infected with witches'broom disease of lime in Oman. Acta Horticulture 928: 375-81.
Amiri Mazraie M., Izadpanah K., Hamzehzarghani H., Salehi M. and Faghihi M. M. 2019. Spread and colonization pattern of ‘Candidatus Phytoplasma aurantifolia’in lime plants [Citrus aurantifolia (Christm.) Swingle] as revealed by real-time PCR assay. Journal of Plant Pathology 101:629-637.
Bagheri A., Salehi M., Faghihi M.M., Samavi S. and Sadeghi A. 2009. Transmission of Candidatus Phytoplasma aurantifolia to Mexican lime by the leafhopper Hishimonus phycitis in Iran. Journal of Plant Pathology 91: S4 105.
Baric S., Berger J., Cainelli C., Kerschbamer C., Letschka T. and Dalla Via J. 2011. Seasonal colonisation of apple trees by ‘Candidatus Phytoplasma mali’revealed by a new quantitative TaqMan real-time PCR approach. European journal of Plant Pathology 129: 455-67.
Bertaccini A. and Duduk B. 2009. Phytoplasma and phytoplasma diseases: a review of recent research. Phytopathologia Mediterranea 48: 355-78.
Bita C. and Gerats T. 2013. Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops. Frontiers in Plant Science 4, 273.
Bové J. 1986. Outbreaks and new records. Oman. Witches' broom disease of lime. FAO Plant Protection Bulletin 34: 217-8
Bové J. M., Danet J. L., Bananej K., Hassanzadeh N., Salehi M. and Garnier M. 2000. Witches’ broom disease of lime (WBDL) in Iran. Fourteenth IOCV Conference, Insect‑Transmitted Procaryotes, pp 207–212
Colhoun J. 1973. Effects of environmental factors on plant disease. Annual Review of Phytopathology 11:343-64.
Dickinson M. 2004. Molecular plant pathology. Garland Science.
Du T., Wang Y., Hu Q. X., Chen J., Liu S., Huang W. J. and Lin M. L. 2005. Transgenic Paulownia expressing shiva‐1 gene has increased resistance to paulownia witches' broom disease. Journal of Integrative Plant Biology 47: 1500-6.
Garnier M., Zreik L. and Bové J. M. 1991. Witches’ broom, a lethal mycoplasmal disease of lime trees in the Sultanate of Oman and the United Arab Emirates. Plant Disease 75:546-551.
Ghini R., Hamada E. and Bettiol W. 2008. Climate change and plant diseases. Scientia Agricola 65. 98-107.
Gundersen D. E. and Lee I. M. 1996. Ultrasensitive detection of phytoplasmas bynested PCR assay using two universal primer pairs. Phytopathol Mediterranea 35:144–151
Hassanzadeh Khankahdani H., Rastegar S., Golein B., Golmohammadi M. and Aboutalebi Jahromi A. 2017. Genetic diversity in Persian lime (Citrus latifolia Tanaka) accessions using morphological and molecular markers. Agriculture and Foresty 63: 221-231.
Hassanzadeh Khankahdani H., Bahrami H. R., Faghihi M. M. and Bagheri A. 2019. Reaction of some commercial citrus species and Iranian lime biotypes to witches’ broom disease of lime. Crop Protection 122: 23-9.
Lee M., Martini M., Marcone C. and Zhu S. F. 2004. Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus Phytoplasma ulmi’for the phytoplasma associated with elm yellows. International Journal of Systematic and Evolutionary Microbiology 54:337–347.
Liu Z., Wang Y., Xiao J., Zhao J. and Liu M. 2014. Identification of genes associated with phytoplasma resistance through suppressive subtraction hybridization in Chinese jujube. Physiological and Molecular Plant Pathology 86: 43-8.
Munyaneza J.E., Sengoda, V. G., Buchman, J.L. and Fisher, T. W. 2012. Effects of temperature on ‘Candidatus Liberibacter solanacearum’and zebra chip potato disease symptom development. Plant Disease 96, 18-23.
Murray M. G. and Thompson W. F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic acids research 8: 4321-6.
Obrępalska-Stęplowska A., Renaut J., Planchon S., Przybylska A., Wieczorek P., Barylsk J. and Palukaitis P. 2015. Effect of temperature on the pathogenesis, accumulation of viral and satellite RNAs and on plant proteome in peanut stunt virus and satellite RNA-infected plants. Frontiers in Plant Science 6: 903.
Rezazadeh N., Asadi Abkenar A. and Rouhibakhsh A. 2019. Reaction assessment of a number of citrus hybrids to ‘Candidatus Phytoplasma aurantifolia’. Iranian Journal of Plant Pathology 54: 173-83.
Salar P., Charenton C., Foissac X. and Malembic-Maher S. 2013. Multiplication kinetics of Flavescence dorée phytoplasma in broad bean. Effect of phytoplasma strain and temperature. European journal of Plant Pathology 135: 371-81.
Salehi Abarghouei E., Izadpanah K., Taghavi S. M., Hamzezarghani H. and Afsharifar A. 2018. Interaction of witches’ broom disease of lime phytoplasma and lettuce phyllody phytoplasma in mixed infection of periwinkle plants. Iranian Journal of Plant Pathology 2: 131-46.
.
Salehi M., Izadpanah K., Siampour M., Bagheri A. and Faghihi S. M 2007. Transmission of ‘Candidatus Phytoplasma aurantifolia’to Bakraee (Citrus reticulata hybrid) by feral Hishimonus phycitis leafhoppers in Iran. Plant Disease 91: 466.
Salehi M., Nejat N., Tavakkoli A. R.and Izadpanah K. 2005. Reaction of citrus cultivars to Candidatus phytoplasma aurantifolla in Iran. Iranian Journal of Plant Pathology 41: 363-73.
Schneider B. and Seemüller E. 1994. Presence of two sets of ribosomal genes in phytopathogenic mollicutes. Applied Environmental Microbiology 60: 3409-12.
Singh A., Permar V., Basavaraj B. S. T. and Praveen S. 2018. Effect of temperature on symptoms expression and viral rna accumulation in groundnut bud necrosis virus infected vigna unguiculata. Iranian Journal of Biotechnology 16: 227-234.
Wang Y., Bao Z., Zhu Y. and Hua J. 2009. Analysis of temperature modulation of plant defenseagainst biotrophic microbes. Molecular Plant-Microbe Interactions 22:498-506.
Zreik L., Carle P., Bov J. M. and Garnier M. 1995. Characterization of the mycoplasmalike organism associated with witches' broom disease of lime and proposition of a Candidatus Taxon for the Organism,“Candidatus Phytoplasma aurantifolia”. International Journal of Systematic and Evolutionary Microbiology 45: 449-453.
Iranian Journal of Plant Pathology
Volume 56, Issue 4
June 2020
Pages 345-358

Files

Share

How to cite

Statistics