Analysis of Pyricularia oryzae isolates from rice and weeds with rep-PCR marker and pathogenicity

Document Type : Research Article

Authors

1 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Assistant professor of Baluchestan Agricultural and Natural Resources Research and Education Center, AREEO, Iranshahr, Iran

Abstract

Pyricularia oryzae is the cause of blast leaf spot disease on rice and more than 50 plant species of crops and weeds in Poaceae family. Sampling was performed in 2017 in rice fields of Guilan and Mazandaran provinces. Morphological studies showed that the isolates obtained from Oryza sativa, Setaria viridis, Echinochloa crus-galli, Sorghum halepense, and Paspalum dilatatum were belonged to P. oryzae. The pathogenicity test revealed that isolates obtained from each host had higher pathogenicity on the same host and were either not pathogenic or less pathogenic on other hosts. Cluster analysis of DNA fingerprinting data using rep-PCR showed that isolates obtained from different hosts were located in three clonal lineages. In general, there were isolates from different hosts in all three identified clonal lineages, and no correlation was found between the similarities of the band pattern with their collection areas.

Keywords

References

Akase K. and Kusaba M. 2017. Overwintering of Pyricularia oryzae in wild infected foxtails. Journal of General Plant Pathology 83:197-204.
Bussaban B., Lumyong S., Seelensa T., Park D. C., Mckenzie E. H. C. and Hyde K. D. 2005. Molecular and Morphological characterization of Pyricularia and allied genera. Mycologia 97:1002-1011.
Chen Q. H., Wang Y. C. and Zheng X. B. 2006. Genetic Diversity of Magnaporthe grisea in China as Revealed by DNA Fingerprint Haplotypes and Pathotypes. Phytopathology 154:361-369.
Consolo V. F., Cordo C. A. and Salerno G. L. 2008. DNA fingerprint and pathotype diversity of Pyricularia oryzae populations from Argentina. Australasian Plant Pathology 37:357-364.
Couch B. C., Fudal I., Lebrun M., Tharreau D., Valent B., Kim P., Notteghem J. and Kohn, M. 2005. Origins of Host-Specific Populations of the Blast Pathogen Magnaporthe oryzae in Crop Domestication with Subsequent Expansion of Pandemic Clones on Rice and Weeds of Rice. Genetics 170:613-630.
Ellis M. B. 1971. Dematiaceous hyphomycetes. Common Wealth Mycological Institute, Kew, England.
Ellis M. B. 1976. Dematiaceous hyphomycetes. Common Wealth Mycological Institute, Kew, England.
Farman M. L. 2002. Pyricularia grisea isolates causing gray leaf spot on perennial ryegrass (Lolium perenne) in the United States: Relationship to P. grisea isolates from other host plants. Phytopathology 92:245-254.
George M. L., Nelson R. G., Ziegler R. S. and Leung H. 1998. Rapid population analysis of Magnaporthe grisea by using rep-PCR and Endogenous repetitive DNA Sequences. Phytopathology 88:223-229.
Javan-Nikkhah M., McDonald B. A. and Banke S. 2004. Genetic structure of Iranian Pyricularia grisea population based on rep-PCR fingerprinting. European Journal of Plant Pathol 110:909-919.
Milazzo J., Pordel A., Ravel S. and Tharreau D. 2019. First scientific report of Pyricularia oryzae causing gray leaf spot disease on perennial ryegrass (Lolium perenne) in France. Plant Disease. 103.
Oh H. S., Tosa Y., Takabayashi N., Nakagawa S., Tomita R., Don L. D., Kusaba M., Nakayashiki H. and Mayama S. 2002. Characterization of an Avena isolate of Magnaporthe grisea and identification of a locus conditioning its specificity on oat. Canadian Journal of Botany 80:1088-1095.
Pordel A., Javan-Nikkhah M., Khodaparast S. A. 2015. A reappraisal of the Pyriculariaceae in Iran. Mycologia Iranica 2:109-116.
Pordel A., Javan-Nikkhah M. and Khodaparast S. A. 2016. Revision of Pyricularia oryzae and occurrence of new hosts for the pathogen Iran. Iranian Journal of Plant Pathology 52:67-83.
Pordel A., Javan-Nikkhah M., Tharreau D., Mirzadi Gohari A. and Moumeni A. 2019. Host-specificity and sexual compatibility of Pyricularia oryzae isolated from different hosts in Iran. Iranian Journal of Plant Pathology 54:277-289.
Pordel A., Ravel S., Charriat F., Gladieux P., Cros-Arteil S., Milazzo J., Adreit H., Javan-Nikkhah M., Mirzadi-Gohari A., Moumeni A., Tharreau D. 2021. Tracing the origin and evolutionary history of Pyricularia oryzae infecting maize and barnyard grass. Phytopathology 111:128-136.
Prabhu A. S., Araujo L. G., Silva G. B. and Trindade M. G. 2007. Virulence and rep-PCR Analysis of Pyricularia grisea Isolates from Two Brazilian Upland Rice Cultivars. Fitopatologia Brasileira. 32:13-70.
Salimi F., Javan-Nikkhah M., Padasht Dehkayi F., Alizadeh A., Soltanlu H. and Yousefirad S. 2018. Dynamic of Pyricularia oryzae at the two stages, leaf, and panicle neck blast based on the assessment of population structure at leaf, tiller, and field levels. Iranian Journal of Plant Protection Science 49:187-201.
Shang J., Wang Y., Su L., Luo M., Yan X., Yu C. and Zhu Y. 2016. Comparative analysis of genetic structure in Magnaporthe oryzae isolates from indica and japonica hosts in China. Gen Plant Pathol.
Shirke M. D., Mahesh H. B. and Gowda M. 2016. Gnome-wide comparison of Magnaporthe species reveals a host-specific pattern of secretory proteins and transposable elements. PLoS one 11:1-19.
Suzuki F., Arai M. and Yamaguchi J. 2006. DNA fingerprinting of Pyricularia grisea by rep-PCR using a single primer based on the terminal inverted repeat from either of the transposable elements Pot2 and MGR586. Journal of General plant Pathology 72:314-317.
Rohlf F. J. 1998. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System version 2.02 User Guide.
Valent B., Farrall L. and Chmley F. G. 1991. Magnaporthe grisea genes for pathogenicity and virulence identified through a Series of Backcrosses. Genetics 127:87-101.
Wicaksono D., Wibowo A. and Widiastatud A. 2017. Genetic Diversity of Pyricularia oryzae, the caudal agent Agent of Rice Blast Disease, Based on Repetitive Element- Based Polymerase chain Reaction. Springer. 2017:41-56.
Xu X., Yang W., Tian K., Zheng J., Liu X., Li K., Lu W., Tan Y., Qin Y. and Wang C. 2018. Genetic diversity and pathogenicity dynamics of Magnaporthe oryzae in the Wuling Mountain erea of China. European Journal of Plant Pathology 153:731-742.
Zarrinnia V., Javan-Nikkhah M., Zamani Zadeh H., Mehrabi R. and Khosravi V. 2011. An investigation of the pathogenicity characteristics of Magnaporthe grisea isolates collected from different host plants from the family Poaceae and race determination of the pathogenic isolates. Iranian Journal of Plant Protection 42:179-190.
Zhong Zh., Norvienyeku J., Chen M., Boa J., Lin L., Chen L., Lin Y., Wu X., Cia Z., Zhang Q., Lin X., Hong Y., Huang J., Xu L., Zhang H., Chen L., Tang W., Zheng H., Chen X., Wang Y., Lian B., Zhang L., Tang H., Lu G., Ebbole D. J., Wang B. and Wang Z. 2016. Directional Selection from Host Plants Is a Major Force Driving Host Specificity in Magnaporthe Species. Scientific Report 6:25591:1-12.
Zhong S. and Steffenson B. J. 2001. Virulence and molecular diversity of Cochliobolus sativus. Phytopathology 91:469-476.
Iranian Journal of Plant Pathology
Volume 57, Issue 1
October 2021
Pages 11-25

Files

Share

How to cite

Statistics