The effect of chitosan on induced resistance against Septoria of wheat

Document Type : Research Article

Authors

1 Assistant professor. Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University

2 Assistant Professor of Department of Horticulture and Crop Research

3 M.Sc. Graduated of Biotechnology

Abstract

The septoria leaf spot caused by Zymoseptoria tritici، is one of the most destructive wheat diseases in the world, which causes lots of damage to this crop annually. The aim of this study was to investigate the effect of 0 and 200 ppm of chitosan on the expression of defense genes and antioxidant enzymes (peroxidase, catalase and ascorbate peroxidase) activity, in Koohdasht wheat cultivar (susceptible cultivar) in interaction with the pathogen. Therefore, two-week plants were sprayed with chitosan, and then treated plants along with control plants were inoculated with Z. tritici pathogen. Finally, the expression rate of PR1, PR2 and PR5 genes were examined using qRT-PCR technique at 0, 12, 24, 48, 96, and 240 hours after inoculation (hai). According to the results, disease severity reduced by about 55% in chitosan pre-treated plants compared to control. The results of qPCR showed that expression of mentioned genes increased in treated and control plants, but the expression of PR1, PR2 and PR5 genes increased by 7.5, 4.15 and 3.76 times more in treated plants compared to control in 24 hai, respectively. Comparing enzyme activity in chitosan pre-treated plants and control, indicates an increasing trend of peroxidase enzyme and a decreasing trend of catalase and ascorbate peroxidase in the first group of plants. The examined gene expression and antioxidants pattern changed in both groups of plants indicates the effectiveness of chitosan in inducing resistance against septoria leaf blotch in wheat.

Keywords


Abdel-Rahman, F.A., Monir, G.A., Hasan, M.S.S., Ahmed, Y., Refaat, M.H., Ismail, I.A. and El-Garhy, H.A.S. 2021. Exogenously Applied Chitosan and Chitosan Nanoparticles Improved Apple Fruit Resistance to Blue Mold, Upregulated Defense-Related Genes Expression, and Maintained Fruit Quality. Hurticulturae. 7: 224-237.
Aranega-Bou P, De la O Leyva, M., Finiti, I., García-Agustín, P. and González-Bosch, C. 2014. Priming of plant resistance by natural compounds. Hexanoic acid as a model. Frontiers in Plant Science. 5:1-12. doi.org/10.3389/fpls.2014.00488
Adhikari, T.B., Anderson, J.M. and Goodwin, S.B. 2003. Identification and molecular mapping of a gene in wheat conferring resistance to Mycosphaerella graminicola. Phytopathology. 93: 1158-1164.
Adhikari, T.B., Balaji, B., Breeden, J. and Goodwin, S.B. 2007. Resistance of wheat to
Mycosphaerella graminicola involve early and late peaks of gene expression. Physiological and Molecular Plant Pathology. 71: 55-68
Aebi, H.E. 1984. Catalase in vitro. Methods Enzymology. 105: 121-126.

Ahangar, L., Babaezad, V., Ranjbar, G.A., Najafi Zarrini, H. 2016. Study of PR gene expression pattern related to in induced resistance to powdery mildew in susceptible wheat genotype after treating with salicylic acid. Journal of crop Breeding. 8: 208-218 (In Persian with English summury)

Amborabe, B.E., Bonmort, J., Fleural-Lessari, P. and Roblin, G. 2008. Early events induced by chitosan on plant cells. Journal of Experimental Botany 59: 2317-2324
Berraies, S., Gharbi, M.S., Belzile, F., Yahyaoui, A., Hajlaoui, M.R., Trifi, M., Jean, M. and Rezgui, S. 2013. High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from   a single wheat field in Tunisia as revealed by SSR markers. African Journal of Biotechnology. 12: 1344-1349.
Collinge, D. and Lyngs Jrgensen, H. 2009. Effects of b-1, 3-glucan from Septoria tritici on structural defense responses in wheat. Journl of Exprimental Botany. 60: 4287-300.
Correa-Aragunde, N., Foresi, N., Delledonne, M., Lamattina, L. 2013. Auxin induces redox regulation of ascorbate peroxidase1 activity by S-nitrosylation/denitrosylation balance resulting in changes of root growth pattern in Arabidopsis. Journal of Experimental Botany. 64: 3339–3349.
Díaz-Martínez, J.M., Aispuro-Hernández, M., Vargas-Arispuro, I.,  Falcón-Rodríguez, A.B. and Martínez-Téllez, M.A. 2018. Chitosan derivatives induce local and distal expression of defence-related genes in wheat (Triticum aestivum L.) seedlings. AGROSIENCIA. 52: 497-509.
Dordas, C. 2009. Role of nutrients in controlling plant diseases. In Lichtfouse, E., Navarrete, M., Debaeke, P., Véronique, S. and Alberola, C. (Ed.), Sustainable Agriculture: A Review. Springer Netherlands. 360-369

Ebrahimi, A., Taliei, F. and Zolfaghari, A. 2020. Effect of salicylic acid and chitosan on response of rice against Fusarium fujikuroi the causal agent of rice root and crown rot. Applied Entomology and Phytopathology. 88: 23-37. (In Persian with English summury)

El-Hadrami, A., Adam, L.R., El Hadrami, I. and Daayf, F. 2010. Chitosan in Plant Protection. Marine Drugs. 8: 968–987. doi: 10.3390/md8040968
El-kereamy, A., El-sharkawy, I., Ramamoorthy, R., Taheri, A., Errampalli, D., Kumar, P. and Jayasankar, S. 2011. Prunusdomestica pathogenesis-related protein-5 activates the defense response pathway andenhances the resistance to fungal infection. PLoS One. 6: e17973. doi: 10.1371/journal.pone.0017973.
Falcón–Rodríguez, A.B., Wégria, G. and Cabrera, J.C. 2012. Exploiting plant innate immunity to protect crops against biotic stress: chitosaccharides as natural and suitable candidates for this purpose. In: Ali, R. Bandani (Eds) New perspectives in plant protection. In Tech. Rikeka, Coratia. 7:139 –166
Felipini, R.B. and Di Piero, R.M. 2013. PR-protein activities in table beet against Cercospora beticola after spraying chitosan or acibenzolar-S-methyl. Tropical Plant Pathology. 38: 534-538.
Frost, C.J., Mescher, M.C., Carlson, J.E. and De Moraes, C. M. 2008. Plant defense priming against herbivores: getting ready for a different battle. Plant Physiology. 146: 818–824. doi: 10.1104/pp.107.113027
Ghazimohseni, V. and Sabbagh, S.K. 2015. Effect of chitosan on gene expression and activity of enzymes involved in resistant induction to fusarium of wheat. Iranian Journal of Plant Protection Science. 46: 363-371 (In Persian with English summury)
Gill, S.S. and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant physiology Biochemical Journal. 48: 909-930.
González, G., Fuentes, L., Moya-León, M.A., Sandoval, C. and Herrera, R. 2013. Characterization of two PR genes from Fragaria chiloensis in response to Botrytis cinerea infection: A comparison with Fragaria x ananassa. Physiological and Molecular Plant Pathology. 82: 73-80
Ha, X., Koopmann, B. and von Tiedemann, A. 2016. Wheat blast and Fusarium head blight display contrasting interaction patterns on ears of wheat genotypes differing in resistance. Phytopathology. 106:270-281.
Harrach, B.D., Fodor, J. and Barna, B. 2005. Changes of antioxidants following powdery mildew infection of near-isogenic barley lines carrying different resistance genes. Proceedings of the 8th Hungarian Congress on Plant Physiology and the 6th Hungarian Conference on Photosynthesis. Acta Biologica Szegediensis. 49: 91-92. 
Hernàndez, H., Figueredo, M. Garrido, N. Sànchez, L. and Sarracent, J. 2005. Intranasal immunisation with a 62 kDa proteinase combined with cholera toxin or CpG adjuvant protects against Trichomonas vaginalis genital tract infections in mice. International Journal for Parasitology. 35: 1333-1337
In, B.Ch., Motomura, Sh., Inamoto, K., Doi, M. and Mori, G. 2007. Multivariente analysis of realation between preharvest environmental factors, postharvest morphological and physiological factors and vase life of cut Asomi Red Roses. Japanese Society for Horticultural Science. 76: 66-72. 
Iriti, M. and Faoro, F. 2004. Plant defense and human nutrition: phenylpropanoids on the menu. Current Topics in Nutraceutical Research. 2: 47-65.
Iriti, M. and Faoro, F. 2009. Chitosan as a MAMP, searching for a PRR. Plant Signal Behavior. 4: 66–68. https://doi.org/10.4161/psb.4.1.7408
Joseph, Sh.M., Krishnamoorthy, S., Paranthaman, R., Moses, J.A. and Anandharamakrishnan, C. 2021. A review on source-specific chemistry, functionality and applications of chitin and chitosan. Carbohydrate Polymer Technologies and Applications. 2: 1-14.
Karimi Farsad, L., Mardi1, M. and Ali Ebrahimi, M. 2013. Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.). Biological Science. 3: 72-78

Khatami, M., Ahangar, L., Taliei, F., Sabouri, H. and Babaezad, V. 2019. Assay of NPR1, MLO and BI-1 genes expression in susceptible wheat to powdery mildew after treatment with chitosan. Journal of Cellular and Molecular Researches. 31: 536-551 (In Persian with English summury)

Kheiri, A., Moosawi Jorf, S.A., Malihipour, A., Saremi, H. and Nikkhah, M. 2016. Application of chitosan and chitosan nanoparticles for the control of Fusarium head blight of wheat (Fusarium graminearum) in vitro and greenhouse. International Journal of biological Macromolecular. 93: 1261-1272. doi: 10.1016/j.ijbiomac.2016.09.072.
Kia, S. and Torabi, M. 2008. Effects of infection with septoria leaf blotch (Septoria tritici) atdifferent growth stages on yield and yield components of wheat cultivars in Gorgan. Seed and Plant. 24: 237-250 (In Persian with English summury).
Kia, Sh. and Sughi, H. 2012. Reaction of Bread Wheat Advanced Genotypes to Mycosphaerella graminicola the Causal Agent of Septoria tritici Leaf Blotch in Greenhouse and Field Conditions. Seed and plant Journal. 28: 133-147 (In Persian with English summury)
Kovács, V., Pál, M., Vida, G., Szalai, G. and Janda, T. 2011. Effect of powdery mildew infection on the antioxidant enzyme activities in different lines of Thatcher-based wheat. Acta Biologica Szegediensis. 55: 99-100.
Livak, K.J. and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real Time Quantitative PCR and the 2-ΔΔCT method. Methods. 25: 402-408.
Maksimov, I.V., Valeev, A.Sh., Cherepanova, E.A. and Yarullina, L.G. 2009. Hydrogen Peroxide Production in Wheat Leaves Infected with the Fungus Septoria nodorum Berk Strains with Different Virulence. Applied Biochemistry and Microbiology. 45: 433–438
Mittler, R., Herr, E.H., Orvar, B.L., van Camp, W., Willekens, H., Inzé, D. and Ellis, B.E. 1999. Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyper responsive to pathogen infection. Procceding Natural Academy Science USA. 96:14165-70.
Modina, L.M., Calibo, C.L. and Borines, L.M. 2009. Antimicrobial property of chitosan and induction of systemic acquired resistance for the control of rice bacterial blight caused by Xanthomonas oryzae pv.oryzae (Swings et al.). Annals of Tropical Research. 31: 69-89.
Mohammadi, M.M., Babaeizad, V., Rahimian, H. and Ebrahim Nejad, Sh. 2017. Screening of some barley lines against powdery mildew agent and considering of NH1 and several Pathogenesis Related genes in disease resistance. Journal of Crop Breeding. 9: 117-124
Moldenhauer, J., Moerschbacher, B.M. and Van der Westhuizen, A.J. 2006. Histological investigation of stripe rust (Puccinia striiformisf. sp. tritici) development in resistant and susceptible wheat cultivars. Plant Pathology. 55: 469-474
Nakano, Y. and Asada, K. 1987. Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiology. 28: 131-140.
Orzali, L., Forin, C. and Riccioni, L. 2014. Effect of chitosan seed treatment as elicitor of resistance to Fusarium graminearum in wheat. Seed Science and Technology. 42: 132-149.
Pieterse, C.M.J., Leon-Reyes, A., Van der Ent, S. and Van Wees, S.C.M. 2009. Networking by small-molecules hormones in plant immunity. Nature Chemical Biology. 5: 308-316.
Pongprayoon, W., Roytrakul, S., Pichayangkura, R. and Chadchawan, S. 2013. The role of hydrogen peroxide in chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.) . Plant Growth Regulation. DOI 10.1007/s10725-013-9789-4
Popova, E.V., Domnina, N.S., Kovalenko, N.M., Sokornova, S.V. and tyuterev, S.L. 2018. Influence of chitosan hybrid derivatives on induced wheat resistance to pathogens with different nutrition strategies. Applied Biochemistry and Microbiology. 54: 535- 539 .
Quaedvlieg, W., Kema, G.H.J., Groenewald, J.Z., Verkley, G.J. M., Seifbarghi, S., Razavi, M., Gohari, A.M. and Mehrabi, R. 2011. Zymoseptoria gen. Nov.: A new genus to accommodate Septoria-like species occurring on graminicolous hosts". Persoonia - Molecular Phylogeny and Evolution of Fungi. 26: 57–69.
Rahneshan, M., Taliei, F., Ahangar, L., Sabouri, H. and Kia, Sh. 2020. Effect of chitosan on the expression pattern of some pathogenicity related genes in wheat infected with powdery mildew. 11: 124-132 (In Persian with English summury)
Sathiyabama, M. and Muthukumar, S. 2020. Chitosan guar nanoparticle preparation and its in vitro antimicrobial activity towards phytopathogens of rice. International Journal of Biological Macromolecules. 153: 297-304.
Sels, J., Mathys, M., De Coninck, B.M.A. and Cammue, B.P.A. 2008. Plant pathogenesis-related (PR) proteins: A focus on PR peptides. Plant Physiology and Biochemistry. 46: 941-950.
Shetty, N.P., Jensen, J.D., Knudsen, A., Finnie, C., Geshi, N., Blennow, A., Collinge, D.B. and Jorgensen, H.J.L. 2009. Effects of beta-1,3-glucan from Septoria tritici on structural defense responses in wheat. Journal of Experimental Botany. 60: 4287-4300
Siddaiah, Ch.N., Harish Prasanth, K.V., Raj Satyanarayana, N., Mudili, V., Gupta, V.K., Kalagatur, N.K., Satyavati, T., Dai, X.F., Chen, J.Y., Mocan, A., Pratap Singh, B. and Srivastava, R.K. 2018. Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation. ScientifiC Reports. 8: 2485. DOI:10.1038/s41598-017-19016-z
Soltanloo, H., Ghadirzade Khorzoghi, E., Ramezanpour, S.S. Kalate Arabi, M. and Pahlavani, M.H. 2010. The expression profile of Chi-1, Glu-2, Glu3 and PR1. 2genes in scab-resistant and susceptible wheat cultivars during infection by Fusarium graminearum. Plant Omics Journal 5:162-166.
Torres, M.A., Jonathan, D.G. and Dangl, J.L. 2006. Reactive oxygen species signaling in response to pathogen. Plant Physiology. 141: 373-378.
Van Loon, L.C., Rep, M. and Pieterse, C.M.J. 2006. Significance of inducible defense-related proteins in infected plants. Phytopathology. 44: 135-162.
Xin, T., Wang, X., Peng, H., Yao, Y., Xie, Ch., Han, Y., Ni, Zh. and Sun, Q. 2012. Transcriptome comparison of susceptible and resistant wheat in response to powdery mildew infection. Genomics Proteomics Bioinformatics. 10: 94-106.
Zhao, J., Daves, L.C. and Verpoorte, R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances. 23: 283-333.