Chickpea chlorotic dwarf virus: natural hosts and genome characterization of an isolate in eastern and southern Iran

Document Type : Research Article

Author

Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Chickpea chlorotic dwarf virus (CpCDV) (Mastrevirus, Geminiviridae) has a wide natural host range. Despite of possessing the potential loss, until now the virus has not been studied in eastern and southern Iran. In this research, natural leguminous hosts of CpCDV in Kerman, Razavi Khorasan and Fars provinces were studied and the full-length genome of a chickpea isolate from Rabor (Kerman province) was characterized. Samples were collected from leguminous plants in different regions of the three aforementioned provinces and the CpCDV infection was tested in PCR. Results showed that leguminous plants including pulse, vegetables, foliage and wild species have been infected with the virus. The full-length genome of the Rabor isolate of CpCDV was sequenced and the resulted sequence was compared with the sequences of similar isolates available in the GenBank. Furthermore, the infectious clone of CpCDV was constructed and agroinoculated into some plants. Thus, the pathogenesis of the virus was demonstrated. Collectively, the results of this study indicated that CpCDV has a wide natural host range in the family Fabaceae. Fenugreek, common sainfoin, alfalfa and sophora are reported as the new hosts of the virus in Iran.

Keywords

References

Akhtar S., Khan A.J. and Briddon R.W. 2014. A distinct strain of Chickpea chlorotic dwarf virus infecting pepper in Oman. Plant Disease 98: 286-286.
Bozorgi N., Heydarnejad J., Kamali M. and Massumi H. 2017. Splicing features in the expression of the complementary-sense genes of Beet curly top Iran virus. Virus Genes 53(2): 323–327.
Boulton M. 2008. Construction of infectious clones for DNA viruses: Mastreviruses. In: Foster G.D., Johansen I.E., Hong Y. and Nagy P.D. (Eds.), Methods in molecular biology 451, plant virology protocols: from viral sequence to protein function. Humana Press, Totowa, pp. 503–523.
Brown J.K., Fauquet, C.M., Briddon R.W., Zerbini M., Moriones E. and Navas-Castillo J. 2012. Geminiviridae. In: King A.M.Q., Adams M.J., Carstens E.B. and Lefkowitz E.J. (Eds.), Virus Taxonomy. Elsevier, San Diego, pp. 351–373.
Farzadfar Sh., Pourrahim R., Golnaraghi A.R. and Ahoonmanesh A. 2008. PCR detection and partial molecular characterization of Chickpea chlorotic dwarf virus in naturally infected sugar beet plants in Iran. Journal of Plant Pathology 90: 247-251.
Grimsley N., Hohn B., Hohn T. and Walden R., 1986. Agroinfection, an alternative route for viral-infection of plants by using the Ti plasmid. Proceedings of the National Academy of Sciences of the United States of America 83: 3282–3286.
Hameed U., Zia-ur-rehman M., Ali A.S., Haider M. and Brown J. 2017. First report of Chickpea chlorotic dwarf virus infecting cucumber in Pakistan. Plant Disease, 101(5): 848.
Hamza M., Tahir M.N., Mustafa R., Kamal H., Khan M.Z., Mansoor S., Briddon R.W. and Amin I. 2018. Identification of a dicot infecting mastrevirus along with alpha-and betasatellite associated with leaf curl disease of spinach (Spinacia oleracea) in Pakistan. Virus Research, 256: 174–182.
Hellens R.P., Edwards E.A., Leyland N.R., Bean S. and Mullineaux P.M. 2000. pGreen: a versatile and flexible binary Ti vector for Agrobacterium mediated plant transformation. Plant Molecular Biology 42: 819–832.
Horn N.M. 1994. Viruses Involved in Chickpea Stunt; Wageningen Agricultural University: Wageningen, The Netherlands, p. 139.
Horn N.M., Reddy S.V. and Reddy D.V.R. 1995. Assessment of yield losses caused by chickpea chlorotic dwarf geminivirus in chickpea (Cicer arietinum) in India. European Journal of Plant Pathology 101: 221–224.
Horn N.M., Reddy S.V., Roberts I.M. and Reddy D.V.R. 1993. Chickpea chlorotic dwarf virus, a new leafhopper transmitted geminivirus of chickpea in India. Annals of Applied Biology 122: 467–479.
Kanakala S. and Kuria P. 2018. Chickpea chlorotic dwarf virus: An emerging monopartite dicot infecting mastrevirus. Viruses: 11(1).
Kraberger S, Kumari S.G., Hamed A.A., Gronenborn B., Thomas J.E., Sharman M., Harkins G.W., Muhire B.M., Martin D.P. and Varsani A. 2015. Molecular diversity of Chickpea chlorotic dwarf virus in Sudan: high rates of intra-species recombination: a driving force in the emergence of new strains. Infection, Genetics and Evolution 29: 203–215.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547-1549.
Makkouk K.M., Fazali Y., Kumari S. and Farzadfar S. 2001. First records of Beet western yellows virus, Chickpea chlorotic dwarf virus, Faba bean necrotic yellows virus and Soybean dwarf virus infecting chickpea and lentil crops in Iran. New Disease Reports 4: 6.
Manzoor M.T., Ilyas M., Shafiq M., Haider M., Shahid A. and Briddon R. 2014. A distinct strain of Chickpea chlorotic dwarf virus (genus Mastrevirus, family Geminiviridae) identified in cotton plants affected by leaf curl disease. Archives of Virology, 159(5): 1217–1221.
Muhire B., Martin D.P., Brown J.K., Navas-Castillo J., Moriones E., Zerbini M., Rivera-Bustamante R.F., Malathi V.G., Briddon R.W. and Varsani A. 2013. A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae). Archives of Virology 158: 1411–1424.
Muhire B.M., Varsani A. and Martin D.P. 2014. SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9 (9): e108277.
Roumagnac P., Lett J.M., Fiallo-Olivé E., Navas-Castillo J., Zerbini F.M., Martin D.P. and Varsani A. 2021. Establishment of five new genera in the family Geminiviridae: Citlodavirus, Maldovirus, Mulcrilevirus, Opunvirus, and Topilevirus. Archives of Virology (In Press).
Shepherd D.N., Martin D.P., Lefeuvre P., Monjane A. L., Owor B.E., Rybicki E.P. and Varsani A. 2008. A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue. Journal of Virological Methods 149: 97-102.
Shahmohammadi N., Dizadji A., Bihamta M.R. and Kvarnheden A. 2020. Diversity and occurrence of chickpea chlorotic dwarf virus on legumes from Iran. Plant Pathology 69:139-148.
Singh K.B. and Saxena M.C. 1999. Chickpeas. In The Tropical Agriculturalist. Macmillan Education Ltd, London. United Kingdom. 134pp.
Young J.M., Kuykendall L.D., Martínez-Romero E., Kerr A., Sawada H. 2001. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. International Journal of Systematic and Evolutionary Microbiology 51:89-103.
Zerbini F.M., Briddon R.W., Idris A., Martin D.P., Moriones E., Navas-Castillo J., Rivera-Bustamante R., Rougmanac P. and Varsani A. 2017. ICTV Report Consortium. ICTV Virus Taxonomy Profile: Geminiviridae. Journal of General Virology 98: 131–133.
Zhang Y.P., Uyemoto J.K. and Kirkpatrick B.C. 1998. A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods 71(1): 45-50.
Zia-Ur-Rehman M., Hameed U., Ali C.A., Haider M.S. and Brown J.K. 2017. First report of Chickpea chlorotic dwarf virus infecting okra in Pakistan. Plant Disease 101: 1336.
Zia-Ur-Rehman M., Hameed U., Herrmann H.W., Iqbal M., Haider M. and Brown J. K. 2015. First report of Chickpea chlorotic dwarf virus infecting tomato crops in Pakistan. Plant Disease 99(9): 1287
Iranian Journal of Plant Pathology
Volume 57, Issue 3
June 2022
Pages 203-216

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