Genome sequence of new isolates of sesame curly top virus in Kerman province and identification of new hosts of the virus

Document Type : Research Article

Authors

1 Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman

2 Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Plant Protection, College of Agriculture, Jiroft University

4 Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman

5 The Biodesign Center of Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and Medicine, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ 85287-5001, USA Structural Biology Research Unit, Department of Clin

Abstract

Sesame curly top virus (SeCTV) is a new species in the genus Turncurtovirus (family Geminiviridae). To date, sesame was the only natural host of the virus. During a survey for identification of geminiviruses infecting crop, vegetable or ornamental plants in south-eastern Iran, the sesame curly top virus infection of alfalfa, bean, eggplant, faba bean, blanket flower, radish and watermelon was confirmed by PCR and sequencing. The full-length genome of seven new SeCTV isolates share >97 and ~87-88% nucleotide identity with other sequences from Iran and Pakistan, respectively. An infectious clone of SeCTV was constructed, biolistically-or Rhizobium-inoculated to several host plant species and the pathogenesis of the virus was demonstrated. Results of this study indicated that, similar to known turncurtoviruses, SeCTV has a wide natural host range in Iran and among the alternative hosts, the rate of infection and severity of disease in watermelon are remarkable.

Keywords

References

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Iranian Journal of Plant Pathology
Volume 57, Issue 4
December 2022
Pages 281-289

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