Antifungal effects of pomegranate extract intercalated into silicate nanoparticles against blue mold of apple

Document Type : Research Article


Department of Plant Protection, College of Aboureihan, University of Tehran


Biological method for controlling (use of plant polyphenols) the postharvest fungi diseases has been suggested as an environmentally friendly method. However the principal problems of plant polyphenols application as fungicide is their rapid decomposition in normal environmental conditions by temperature, oxygen and light. One of the best solutions for this problem is encapsulation or intercalation them into nanoparticles In this regard, the pomegranate fruit peel and clove rich of polyphenols was extracted by water and methanol and was intercalated into the layers of silica nanoparticles (montmorillonite) and his antifungal effect was tested  via broth dilution method and agar well-diffusion method against blue mold fungi. The XRD, FTIR and DLS analyses confirmed completely intercalation of pomegranate peel extract into interlayer of montmorillonite. The results showed that in the broth dilution method, nanosilica particles alone had antifungal effect similar to commercial fungicide. And intercalation extract into nanoparticles did not affect its antifungal effects. In the contrast,, in agar well-diffusion method, intercalation the extract into nanoparticles increased its  inhibitory effect  very significantly (p <0.001) compared to nanoparticles and extract alone (as much as 26%). This effects was apart from the improved stability of plant extracts containing polyphenols in the storage conditions. These results showed that intercalation the polyphenols of pomegranate fruit peel into silica nanoparticles, could be a environmentally friendly, eco-friendly and economic method for controlling the postharvest blue mold fungi instead of chemical fungicides that have many environmental hazards for humans, animals and soil.