Molecular characterization of defense-related genes, induced against Ralstonia solanacearum in potato

Document Type : Research Article

Authors

1 Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

2 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Ka-raj, Iran.

3 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Abstract

Molecular responses of susceptible (Marfona) and tolerant (Els) cultivars of Solanum tuberosum, and a resistant genotype of S. phureja were comparatively studied using a cDNA-AFLP technique to identify candidate genes involved in defense mechanisms against Ralstonia solanacearum. Transcriptional changes were studied at 0, 2, 4 and 7 days post inoculation of the plants by R. solanacearum through root inoculation under in vitro conditions. Of the 2820 transcript derived fragments (TDFs) detected, 206 TDFs showed variable patterns. In total 20 TDFs with stronger differential induction were sequenced. These TDFs showed significant homologies with the genes involved in physiological processes, stress responses, cell structure, defense, transcription and protein synthesis/folding. Approximately 5% of the sequenced TDFs were identified with unknown function. Induction of a higher number of genes was altered in response to R. solanacearum in S. phureja compared to S. tuberosum.

Keywords

References

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Iranian Journal of Plant Pathology
Volume 54, Issue 3
March 2019
Pages 163-172

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