Rapid detection of Potato virus S using antibody-coated gold nanoparticles

Document Type : Research Article

Authors

faculty member

Abstract

In spite of existing of several methods for detection of viruses, time consuming is major limitation of them. So, developing faster and real time method is important to certificate the plant materials in quarantine stations and virus management. In the recent research, nanotechnology was employed to detect Potato virus S (PVS) using gold nanoparticles (AuNPs). Colloidal AuNPs were prepared through citrate reduction and conjugated to a specific antibody against PVS coat protein. Conjugation was optimized by changing the pH of AuNPs and antibody concentration. Antibody coated nanoparticles were used to detect different concentrations of antigen using spectrophotometry. After binding the antibody to the gold nanoparticles which cause an increase in particle size, the SPR peak was displaced in range of 4-5 nm and shifted from 524 to 530 nm. The binding of the antigen to the nanoparticle also caused a change in the particle size and an increase maximum absorbance from 530 to 539 nm. No spectral change were seen when PVY and CMV used as controls. This study is the first attempt of nanoparticle usage in quick and easy detection of PVS

Keywords

References

Ali M. A., Salaheldin T. A., Moghazy G. M. E., Tork I. M. and Omara I. I. 2014. Detection of E.coli O157:H7 in feed samples using gold nanoparticles sensor. International Journal of Current Microbiology and Applied Sciences 3(6): 697-708.
Choi D. S., Jin H. E., Yoo S. Y. and Lee S. W. 2014. Bioconjugate Chem 25: 216-223.
Cox B. A. and Jones R. A. C. 2010. Genetic variability in the coat protein gene of Potato virus S isolates and distinguishing its biologically distinct strains. Archive of Virology 155: 1163-1169.
Danks C. and Barker I. 2000. On-site detection of plant pathogens using lateral-flow devices. OEPP/EPPO Bulletin 30: 421-426.
Davatgaran-Taghipour Y., Kharrazi S. and Amini S.M. 2018. Antibody Conjugated AuNPss for Detection of Small Amounts of Antigen Based on Surface Plasmon Resonance (SPR) Spectra. Nanomedicine Research Journal 3(2): 102-108.
Dong-Pham V., Hoang H., Hoang Phan T., Conrad U. and Ha Chu H. 2012. Production of antibody labeled gold nanoparticles for influenza virus H5N1 diagnosis kit development. Advances in Natural Sciences: Nanoscience and Nanotechnology 3: 17(7pp).
Fatemi F., Amini S. M., Kharazi S., Rasaee M. J., Mazlomi M. A., Asadi-Ghaleni M., Rajabibaz M. and Sadroddiny E. 2017. Construction of genetically engineered M13K07 helper phage for simultaneous phage display of gold binding peptide 1 and nuclear matrix protein 22 ScFv antibody. Colloids and Surfaces B: Biointerfaces 159: 770-780.
Huang X. 2006. Gold nanoparticles used in cancer cell diagnostics, Selective photothermal therapy and catalysis of NADH oxidation reaction. PhD. thesis. Georgia Institute of Technology P. 143.
King A. M. Q., Lefkowitz E. J., Mushegian A. R., Adams M. J., Dutilh B. E., Gorbalenya A. E., Harrach B., Harrison R. L., Junglen S., Knowles N. J., Kropinski A. M., Krupovic M., Kuhn J. H., Nibert M. L., Rubino L., Sabanadzovic S., Sanfaçon H., Siddell S. G., Simmonds P., Varsani A., Zerbini F. M. and Davison A. J. 2018. Changes to taxonomy and the International Code of Virus Classifcation and Nomenclature ratifed by the International Committee on Taxonomy of Viruses (2018). Archives of Virology 163: 2601-2631.
Lai Y. H., Koo S., Oh S. H., Driskell E. A. and Driskell J. D. 2015. Rapid screening of antibody–antigen binding using dynamic light scattering (DLS) and gold nanoparticles. Analytical Methods 7(17): 7249-55.
Larossi M., Schiattarella C. H., Rea I., De Stefano L., Fittipaldi R., Vecchione A., Velotta R. and Delta Ventura B. 2018. Colorimetric Immunosensor by Aggregation of Photochemically Functionalized Gold Nanoparticles. ACS Omega 3: 3805-3812.
Ling K. S., Zhu H. Y., Jiang Z. Y. and Gonsalves D. 2000. Effective application of DAS-ELISA for detection of grapevine leafroll associated closterovirus-3 using a polyclonal antiserum developed from recombinant coat protein. European Journal of Plant Pathology 106: 301-309.
Liu P., Yang X. and Sun S. 2013. Enzyme-free colorimetric detection of DNA by using gold nanoparticles and hybridization chain reaction amplification. Analytical Chemistry 85(16): 7689-7695.
Masoudi N., Rouhibakhsh A., Asare M. H., Naderpour M. and Koolivand D. 2019. Identification of dominant isolate of Potato virus S in Iran and heterologous expression of its coat protein. Genetic Engineering and Biosafety Journal 7 (2): 153-162.
Matousek J., Schubert J., Ptacek J., Kozlova P. and Dedic P. 2005. Complete nucleotide sequence and molecular probing of Potato virus S genome. Acta Virologica 49: 195-205.
O’Sullivan M. J., Bridges J. W. and Marks V. 1979. Enzyme Immunoassay: A Review. Annals of Clinical Biochemistry: An International Journal of Biochemistry and Laboratory Medicine 16 (1-6): 221-39.
Perenboom J. A. A. J., Wyder P. and Meier F. 1981. Physics Reports 78: 173-292.
Perezjuste J. 2005. Gold nanorods: Synthesis, characterization and applications. Coordination Chemistry Reviews 249 (17-18): 1870-1901.
Petryayeva E. and Krull U. J. 2011. Localized surface plasmon resonance: Nanostructures, bioassays and biosensing. Areview. Analytica Chimica Acta 706 (1): 8-24.
 
Ricci F., Volpe G., Micheli L. and Palleschi G. 2007. A review on novel developments and applications of immunosensors in food analysis. Analytica Chimica Acta 605: 111-129.
Saleh M., Soliman H., Haenen O. and El.Matbouli M. 2011. Antibody-coated gold nanoparticles immunoassay for direct detection of Aeromonas salmonicida in fish tissues. Journal of Fish Diseases 34: 845-852.
Salomone A. and Roggero P. 2002. Host range, seed transmission and detection by ELISA and lateral flow of an Italian isolate of Pepino mosaic virus. Journal of Plant Pathology 84: 65-68.
Salomone A., Bruzzone C., Minuto G., Minuto A. and Roggero P. 2002. A comparison of lateral flow and ELISA for the detection of Tomato mosaic virus in tomato. Journal of Plant Pathology 84: 193.
 
Slot J. W. and Geuze H. J. 1985. A new method of preparing gold probes for multiple labeling cytochemistry. European Journal of Cell Biology 38: 87-93.
Springer T. and Homola J. 2012. Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma. Analytical and Bioanalytical Chemistry 404 (10): 2869-75.
Turkevich J., Stevenson P. C. and Hillier J. 1951. A study of the nucleation and growth processes in the synthesis of colloidal gold. Discussions of the Faraday Society 11-55.
Voller A., Bidwell D. and Bartlett A. 1976. Enzyme immunoassays in diagnostic medicine: Theory and practice*. Bulletin of the World Health Organization 53 (1): 55.
Xiulan S., Xiaolian Z., Jian T., Zhou J. and Chu F. S. 2005. Preparation of gold-labeled antibody probe and its use in immunochromatography assay for detection of aflatoxin B1. International Journal of Food Microbiology 99: 185-194.
Zimmermann D., Bass P., Legin R. and Walter B. 1990. Characterization and serological detection of four closterovirus-like particles associated with leafroll disease on grapevine. Journal of Phytopathology 130: 205-218.
Iranian Journal of Plant Pathology
Volume 55, Issue 2
December 2019
Pages 105-114

Files

Share

How to cite

Statistics