Identification and detection of Phytophthora erythroseptica and its hybrids using specific primers

Document Type : Research Article

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Abstract

Phytophthora erythroseptica, the causal agent of potato pink rot, is one of the oomycete plant pathogens that causes significant losses in field and storage. In order to develop a sensitive and rapid method for detection and identification of P. erythroseptica, six nuclear and mitochondrial gene regions were investigated to design species-specific primers. Due to the high similarity of P. erythroseptica sequences to its closely related species, only one nuclear region, TigA, was appropriate to design specific primers. Using specific primers, a simple as well as a nested-PCR based method was developed for the identification and detection of P. erythroseptica. The specificity of designed primers was examined using a collection of Phytophthora species from different phylogenetic clades as well as close relatives of P. erythroseptica. In addition to pure DNA, designed primers detected P. erythroseptica in infected plant tissues including potato, tomato and spinach. Specific primers detected 10 pg of p < /em>. erythroseptica pure DNA, however, nested PCR increased primers sensitivity at least 100 times. Moreover, specific primers designed in this study were able to detect P. erythroseptica as the maternal or paternal parent species in hybrid isolates that would make a significant help to recognize one of the parental species in hybrids of P. erythroseptica.

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Iranian Journal of Plant Pathology
Volume 55, Issue 3
February 2020
Pages 243-259

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