Effect of arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices and antagonistic fungi Pochonia chlamydosporia var. chlamydosporia and Purpureocillium lilacinum on disease indices and reproduction rate of Meloidogyne javanica in three peach rootstocks under greenhouse conditions

Document Type : Research Article

Authors

1 -

2 Associate Prof. for plant pathology, Plant Protection Dep., Faculty of Science and Agricultural Engineering. University of shhrekord

3 Dept. of plant protection-faculty of agriculture- Zabol University

4 Dept.of plant protection-faculty of agriculture-Zabol university

5 Ministry of Science -tehran

Abstract

Biocontrol is one of the methods widely studied to reduce nematode damage. The biological control efficacy of two arbuscular mycorrhizal fungi, Glomus mosseae and G. intraradices and two antagonistic fungi, Pochonia chlamydosporia var. chlamydosporia and Purpureocillium lilacinum on disease indices and reproduction rate of root-knot nematode, Meloidogyne javanica were studied on three local cultivars of peach rootstocks namely Helendri, Shorabi3 and Gf677. The experiment was conducted as a factorial experiment in a completely randomized design with 30 treatments and five replications under greenhouse conditions. Evaluations were performed 90 days after nematode inoculation by measuring the plant growth parameters and growth and reproduction rate of M. javanica. Results of nematode reproduction indices showed more effect of P. chlamydosporia var. chlamydosporia on reduction of nematode biological activity than other biocontrol fungi. The number of galls, egg masses, second stage juveniles and reproduction factor in GF677 treated with this fungus decreased comparing to control, as 42.2, 41.8, 39 and 6.36 percent, respectively. Although the fungus was effective on all stages of nematode development, but the number of egg masses and number of eggs per egg mass were more affected.

Keywords

References

Abd El-Raheem R. S., Samir A. S., Yehia A. G. M. and Doaa M. K. 2005. Evaluation of Pochonia chlamydosporia, Paecilomyces lilacinus and Arthrobotrys dactyloide as biocontrol agents for Meloidogyne incognita under greenhouse condition. Pakistan Journal of Biological Sciences 8 (11): 1511-1516.
Barea J. M., Azcon R. and Azcon-Aguilar C. 2002. Mycorrhizosphere interactions to improve plant fitness and soil quality. Antonie Van Leeuwenhoek 81: 343-351.
Berg R. H. and Tylor C. G. 2008. Cell biology of plant nematode parasitism. Heidelberg. Germany. 273 p.                                                                
Calvet C., Pinochet J., Hernández-Dorrego A., Estaún V. and Camprubí A. 2001. Field microplot performance of the peach-almond hybrid GF-677 after inoculation with arbuscular mycorrhizal fungi in a replant soil infested with root-knot nematodes. Mycorrhiza 10: 295–300.
Chen S. Y. and Dickson D. W. 2004. Biological control of nematodes by fungal antagonists. In: Chen Z. X, Chen S. Y., and Dickson D. W. (eds.), Nematology, Advances and Perspectives-Vol. 2. Nematode management and utilization. CABI Publishing, Wallingford, UK. pp: 979-1039.
Davies K. G. and Spiegel Y. 2011. Biological control of plant-parasitic nematodes: Building coherence between microbial ecology and molecular mechanisms, Progress in Biological Control 11. Springer Science+Business Media, Dordrecht.
De Leij F. A. A. M. and Kerry B. R. 1991. The nematophagous fungus Verticillium chlamydosporium as a potential biological control agent for Meloidogyne arenaria. Revue de Nématologie 14: 157–64.
De Leij F. A. A. M., Kerry B. R. and Dennehy J. A. 1993. Verticillium chlamydosporium as a biological control agent for Meloidogyne incognita and M. hapla in pot and microplot tests. Nematologica 39: 115-126.
Dong K., Dean R. A., Fortnum B. A. and Lewis S. A. 2001. Development of PCR primer to identify species of root-knot nematode: Meloidogyne arenaria, M. hapla, M. incognita and M. javanica. Nematropica 31: 273-282.
Faostat. 2012. Available in http://faostat.fao.org/
Ganaie M. A. and Ahmadkhan T. 2010. Biological potential of Paecilomyces lilacinus on pathogenesis of Meliodogyne javanica infecting tomato plant. European Journal of Applied science 2: 80-84.
Hussey R. S. and Barker K. R. 1973. Comparison of methods for collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter 57: 1025–1028.
Jatala P. 1986. Biological Control of plant parasitic nematodes. Annual Review of Phytopathology 24: 453-489.
Jones J. T., Haegeman A., Danchin E. G., Gaur H. S., Helder J., Jones M. G., Kikuchi T., Manzanillia-Lopez R., Palomares-Rius J. E., Wesemael W. M., and Perry R. N. 2013. Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology 14: 946-961.
Kerry B. R., Simon A. and Rovira A. D. 1984. Observations on the introduction of Verticillium chlamydosporium and other parasitic fungi into soil for control of the cereal cyst-nematode Heterodera avenae. Annals of Applied Biology 105: 509–516.
Linderman R. G. 2000. Effects of mycorrhizas on plant tolerance to disease. Arbuscular Mycorrhizas: Physiology and Function. pp: 345-367.
Mckenry M. V. 1985. Integrated pest management for almond. University of California, Division of agriculture and natural resources, publication 3308.
Menge J. A., Powell C. L. and Bagyaraj D. P. 1984. Inoculum production. In: C. L. Powell and D. P. Bagyaraj (Eds.). VA Mycorrhiza. CRC Press Inc., Boca Raton, Florida, USA pp 187-199.
Moosavi M. R., Zare R., Zamanizadeh H. R. and Fatemy S. 2010. Pathogenicity of Pochoniaspecies on eggs of Meloidogyne javanica. Invertebrate Pathology 104: 125- 133.
Moosavi M. R. and Zare R. 2015. Factors affecting commercial success of biocontrol agents of phytonematodes. In: T. H. Askary & P. R. P. Martinelli (Eds.), Biocontrol agents of phytonematodes. (pp 187–202) CABI Publishing.
Nyczepir A. P., Riley M. B and. Sharpe R. R. 1993. Dynamics of concomitant populations of Meloidogyne incognita and Criconemella xenaplax on peach. Journal of Nematology 25: 659-665.
Oostenbrink M. 1966. Major characteristics of the relation between nematodes and plants. Mededelingen Landbouwhogeschool Wageningen 66 (4): 1- 46.
Oyekanmi E. O., Coyneb D. L., Fagadea O. E. and Osonubia O. 2007. Improving root-knot nematode management on two soybean genotypes through the application of Bradyrhizobium japonicum, Trichoderma pseudokoningii and Glomus mosseae in full factorial combinations. Crop Protection 26: 1006–1012.
Pandey S. 2013. A Study on the interaction effect of various interaction levels of VAM fungi with phytoparasitic cyst nematode H. cajani infecting cowpea. Indian Journal of Fundamental and Applied Life Sciences 3 (1): 203-207.
Phillips J. M. and Hayman D. S. 1974. Improved procedures clearing root and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infections. Transaction of British Mycological Society 55: 158-161.
Shreenivasa K. R., Krishnappa K. and Ravichandra N. G. 2006. Interaction effects of arbuscular mycorrhizal fungus Glomus fasciculatum and root-Knot nematode, Meloidogyne incognita on growth and phosphorous uptake of tomato. Karnataka Journal of Agricultural Sciences 20: 57 – 61.
Silva A. T., Penna J. C. V., Goulart L. R., Santos M. A. and Arantes N. E. 2000. Genetic variability among and within races of Heterodera glycines ichinohe assessed by RAPD markers. Genetics and Molecular Biology 23: 323-32.
Sohrabi F., Sheikholeslami M., Heydari R., Rezaee S. and Sharifi R. 2017. Study on combined application of arbuscular mycorrhizal fungi isolates and plant growth promoting rhizobacteriain controlling root-knot nematode Meloidogyne javanica in tomato under greenhouse conditions. Iranian Journal of Plant pathology 53 (4): 449-462.
Strobel N. E. 1982. Interactions of vesicular-arbuscular mycorrhizal fungi Meloidogyne incognita and soil fertility on peach. Phytopathology 72: 690-694.
Taylor D. P. and Netscher C. 1974. An improved technique for preparing perineal patterns of Meloidogyne spp. Nematologica 20: 268-269.
Tikhonov V. E., Lopez-Llorca L. V., Salinas J. and Jansson H. B. 2002. Purification and characterization of chitinases from the nematophagous fungi Verticillium chlamydosporium and V. suchlasporium. Fungal Genetics and Biology 35: 67–78.
Rezaee Danesh Y., Mohammadi Goltapeh A., Alizadeh A. and Varma A. 2007. Studies on taxonomy and in vitro culturing possibility of soybean and alfalfa-associated arbuscular mycorrhizas in Iran. Ph.D thesis. Plant protection department, Faculty of agriculture. Tarbiat Modarres university 346 p.
Roserwarne G. M., Barker S. J. and Smith S. E. 1997. Production of near-synchronous fungal colonization in tomato for developmental and molecular analyses of mycorrhiza. Mycological Research 101: 966–970.
Rumbos C., Reimann S., Kiewnick S. and Richard A. 2009. Interactions of Paecilomyces lilacinus strain 251 with the mycorrhizal fungus Glomus intraradices. Implications for Meloidogyne incognita control in tomato. Biocontrol Science and Technology 16 (9): 981-986.
Waceke J. V., Waudo S. V. and Sikora R. 2001. Suppression of Meloidogyne hapla by arbuscular mycorrhizal fungi on pyrethrum in Kenya. Pest Management Science 47: 135-14.
Zijlstra C., Donkers Venne D. and Fargette M. 2000. Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterized amplified region (SCAR) based PCR assays. Nematology 2: 847-853.
Iranian Journal of Plant Pathology
Volume 56, Issue 2
November 2020
Pages 163-175

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