Investigating the interaction of some mutated strains of Trichoderma species and Piriformospora indica on Cucumber wilt caused by Fusarium in greenhouse

Document Type : Research Article

Authors

1 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI)

2 Plant Protection Department, Sari University of Agricultural Sciences and Natural Resources, Mazandaran, Iran

Abstract

The aim of this study is to investigate the interaction effects of two Trichoderma spices (T.koningii and T. virens) mutants and endophyte Piriformospora indica on infected cucumber plants by Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) and evaluating their mutual influence on growth indexes in cucumber plants.The efficient wild-type Trichoderma isolates were selected by dual-culture test (T.koningii NAS-108 with 50% and T.virens NAS-115 with 33% inhibition of pathogenic growth) and their spore suspension with a concentration of 106 (spore/ml) in a dose range of 250 Gy was irradiated with gamma rays. NARS-TKM101 and NARS-TviM112 mutants showed highest antagonistic activity by 20% and 100% increase compared to their wild-type strains and they with their wild-type strains, were used for greenhouse evaluations. The combined treatments of Trichoderama sp. (total wild types and total mutants) with P. indica showed the most positive effects on seedlings growth indices length and dry weight (stem and root). No significant difference was observed between un-irradiated and mutant strains of Trichoderma in reducing disease index, (p<5%), but the combined treatments of two Trichoderma fungi (total mixed Trichoderama wild types and total mutants) with P. indica led to significant reduction of disease index (p<5%) and were more successful than the carbendazim fungicide in the plants infected with Forc.

Keywords

References

 ABBASI, S., N. SAFAIE and M. SHAMSBAKHSH, 2014. Evaluation of gamma-induced mutants of Trichoderma harzianum for biological control of charcoal rot of melon. (Macrophomina phaseolina) in laboratory and greenhouse conditions. Journal of Crop Protection 3, 4: 509-521.
 
ABBASI, S., N. SAFAIE and M. SHAMSBAKHSH and SHAHBAZI, S., 2016. Bio control activities of gamma induced mutants of Trichoderma harzianum against some soil borne fungal pathogens and their DNA fingerprinting. Iranian journal of biotechnology, 14(4), p.260.
 
ABDEL-LATEIF, K.S, 2017. Trichoderma as biological control weapon against soil borne plant pathogens. African Journal of Biotechnology, 50: 2299-2306.
 
AFIFY, A.E.M.R., A.A.E. MOHAMED, M.I. GHADA and W.K. BASSAM, 2013. Exposing of Trichoderma spp. to Gamma Radiation for Stimulating Pesticide Biodegradation Activity. J. Plant Pathol. Microb, 4(9) : 1-5. ISSN:2157-7471.
 
AKHLIKY M. R., MORADZADEH ESKANDARI M., ROUHANI H. and MAHDIKHANI MOGHADAM, I., Investigating the efficiency of commercial and native Trichoderma isolates in controlling Fusarium wilt disease of cucumber, The 22nd Iranian Plant Pathology Congress, 2015 in persian.
 
AKRAMI, M, 2015. Effects of Trichoderma spp. in bio-controlling Fusarium solani and F. oxysporum of cucumber (Cucumissativus). Journal of Applied Environmental and Biological Sciences, 4(3): 241-245.
 
ANITH, K. N., FASEEELA, K. M., ARCHANA, P. A., & PRETHAPAN, K. D.. Compatibility of Piriformospora indica and Trichoderma harzianum as dual inoculants in black pepper (Piper nigrum L.). 2011. Symbiosis, 55, 11-17.‏
 
AWAD, H. M, 2004. Studies on Root Rot Diseases of Pea. M.Sc. thesis, Faculty of Agriculture, Minufiya University.
 
BAHARVAND, A., S. SHAHBAZI, H. AFSHARMANESH and M. ALI, 2014. Investigation of gamma irradiation on morphological characteristics and antagonist potential of Trichoderma viride against M. phaseolina. International Journal of Farming and Allied Sciences, 3(11): 1157-1164.
 
 COHEN, R., ORGIL G., BURGER, Y., SAAR, U., ELKABETZ, M. and Tadmor Y, 2015. Differences in the responses of melon accessions to Fusarium root and stem rot and their colonization by Fusarium oxysporum f. sp. radiciscucumerinum. Plant Pathology 64:655–663.
 
DESHMUKH, S, 2006. The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley. Proc. Natl. Acad. Sci. 103: 18450-18457.
 
DI PIETRO, A., M.P. MADRID, Z. CARACUEL, J DELGADO-JARANA and M.I.G. RONCERO, 2003. Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Molecular Plant Pathology: 4: 315–325.
 
ERCOLE, N. and S. GENNARI, 1993. Biological Control of Fusarium Wilt of Melon by Seed Coating with Trichoderma harzianum. Rifai Culture Protecte, 22: 4-73.
 
FEYSIA, B., A. LENCHO., T. SELVARAJ and G. GETANEH, 2015. Evaluation of some botanicals and Trichoderma harzianum for the management of tomato root-knot nematode (Meloidogyne incognita (Kofoid and White) Chit Wood). Advances in Crop Science and Technology, 4(1): 2-10.
 
GAMS, W. and J. BISSET, 1998. Morphology and identification of Trichoderma. In Trichoderma and Gliocladium. Vol. 1.Basic Biology, Taxonomy and Genetics (C. P. Kubicek & G. E. Harman, eds): 3–34. Taylor & Francis, London.
 
GOHARZAD, F., T. GHANBARY, S. SHAHBAZI and H. ASKARI, 2019. Two–dimensional and chitinase activity analysis of a novel mutant of Trichoderma koningii for biodegradation of Macrophomina phaseolina cell walls. Mycologia Iranica, 6(2): 73-87.
 
HAGGAG, W.M. and H.A.A., MOAHAMED, 2002. Enhancement of antifungal metabolite production from gamma-ray induced mutants of some Trichoderma species for control onion white disease. Plant Pathology Bulletin, 11: 45-56.
 
HARMAN, G. E, 2011. Multifunctional fungal plant symbiont: new tools to enhance plant growth and productivity. New Phytol, 189: 647–649.
LIU, L., J.W. KLOEPPER and S. TUZUN, 1995. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology, 85(6): 695-698.
 
MISHRA, V.K, 2010. In vitro antagonism of Trichoderma species against. Pythium aphanidermatum. J. Phytol, 2: 28–35.
 
MOHAMADI, A. S., S. SHAHBAZI and H. ASKARI, 2014. Investigation of γ-radiation on morphological characteristics and antagonist potential of Trichoderma viride against Rhizoctonia solani. International Research Journal of Applied and Basic Sciences 8, 3: 329-336.
 
MOHAMMADI KASHKA F., PIRDASHTI H., YAGHOUBIAN Y., BAHARI SARAVI S.H., Effect of Trichoderma virens and Piriformospora indica coexistence with Enterobacter sp. on the growth and photosynthetic pigments of pepper (Capsicum annuum L.) plant, 2015, Plant Ecophysiology, Vol 8, No 6, in persian.
 
MORADI, R., S. SHAHBAZI, and H. ASKARI, 2015. The impact of γ-radiation on morphological characteristics and antagonist potential of Trichoderma harzianum against Fusarium solani. Journal homepage: www. Isi journal. Info, (4): 7.
 
MUKHERJEE, P.K., P. D. SHERKHANE and N.B. MUTHY, 1999. Induction of stable Benomyl-tolerant phenotypic mutants of Trichoderma pseudokoningii MTCC 3011, and their evaluation for antagonistic and bio control potential. Indian J. Exp. Biol. 37: 710-712 .
 
MUKHERJEE, M., B. A.  HORWITZ, P.D. SHERKHANE, R. HADAR and P.K. MUKHERJEE, 2006. A secondary metabolite biosynthesis cluster in Trichoderma virens: evidence from analysis of genes under expressed in a mutant defective in morphogenesis and antibiotic production. Current genetics 50, 3: 193-202.
 
REDDA, E. T., J. MA, J. MEI, M. LI, B. Y. WU and X. JIANG, 2018. Biological Control of Soilborne Pathogens (Fusarium oxysporum f. sp. cucumerinum) of Cucumber (Cucumis sativus) by Trichoderma sp. Journal of Life Sciences, 12: 1-12.
 
SAHAMPOUR, L., F. ZAKER TAVALLAIE, S. R. FANI and S. SHAHBAZI, 2020.  In vitro efficiency of Trichoderma harzianum mutants in biocontrol of Fusarium oxysporum f. sp. radicis-cucumerinum. Journal of Crop Protection. 9(2): 285-300.
 
SEPEHRI, M., N. SALEH RASTIN, GH. HOSSEINI SALKADEH and M. KHAYAM NEKOUEI, 2009. Effect of endophytic fungi, Piriformospora indica, on growth and resistance of Hordeum Vulgarei. to salinity stress. Rangeland, 3(3): 508-518. (Persian with English summary).
 
SHAHBAZI, S., H. ASKARI and S. MOJERLOU, 2016. The impact of different physicochemical parameters of fermentation on extracellular cellulolytic enzyme production by Trichoderma harzianum.  5(3): 397-412.
 
SHAHRIARI, D. and R. ZARE, 2006. Fusarium stem and root rot of greenhouse cucumber. Proceedings of the 17th Iranian Plant Protection Congress, Karaj, Iran (in Persian).
 
TAPWAL, A., G. THAKUR, S. CHANDRA and A. TYAGI, 2015. In-vitro evaluation of Trichoderma species against seed borne pathogens. Int J Biol Chem Sci, 1(10): 14-19.
 
VAN WEES, S. C. M., S. VAN DER ENT and C. M. J PIETERSE, 2008. Plant immune responses triggered by beneficial microbes. Curr Opin Plant Biol. 11: 443–448.
 
VARMA, A., S. VERMA, S. N. SUDAH, P. Franken, 1999 Piriformospora indica, a cultivable plant growth-promoting root endophyte. Appl Environ Microbiol 65: 2741–2744.
 
WAGH, S., S.T. INGLE, S. DANDALE and S.S. MANE, 2015. Improvement in bio control ability of Trichoderma viride through gamma irradiation. Trends in Biosciences, 8(20): 5622-5626.
 
WOO, S.L., M. RUOCCO, F. VINALE, M. NIGRO, R. MARRA, N. LOMBARDI, A. PASCALE, S. LANZUISE, G. MANGANIELLO and M. LORITO, 2014. Trichoderma-based products and their widespread use in agriculture. The Open Mycology Journal, 8(1): 71-126.
 
YADIDIA, I., A.K. SRIVASTVA, Y. KAPULNIK and I. CHET, 2001. Effect of Trichoderma harizanum on microelement concentrations and increased growth of cucumber plants. Plant and Soil, 235: 235-242.
Iranian Journal of Plant Pathology
Volume 58, Issue 2
September 2023
Pages 77-95

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