Effect of seed bio-/chemo-priming on Rhizoctonia root rot of common bean (Phaseolus vulgaris L.)

Document Type : Research Article

Authors

1 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Tarbiat Modares University

Abstract

Rhizoctonia root rot disease of common bean (Phaseolus vulgaris L.), caused by Rhizoctonia solani AG4, is one of the most important bean diseases worldwide. The use of biological and non-biological prime agents has become a promising and environmentally friendly alternative to the management of soil-borne pathogens. Therefore, the present study aimed to compare the effect of Bacillus subtilis isolate A4 (biological prime) and beta-aminobutyric acid (BABA) (non-biological prime) on the management of Rhizoctonia root rot disease in Talash and Sadri cultivars of common bean plants. The seeds were coated with the mentioned priming agents and after drying, they were planted and kept in the greenhouse at a temperature of 20±2 °C. Plants at the cotyledon stage were inoculated by R. solani AG4 inoculum. The induction of resistance in plants was evaluated by measuring disease severity, morphological, and physiological factors three weeks after inoculation. The priming factors increased almost all morphological and physiological measured traits, compared to the control. The plants treated with prime agents had a lower disease severity index (DSI) compared to the control plants and the Talash cultivar treated with B. subtilis had the lowest DSI, with a 59% reduction. The phenol content, catalase and peroxidase enzyme activities under bio-priming and chemo-priming treatments in the presence of the pathogen showed a significant increase compared to the control plants.

Keywords

Iranian Journal of Plant Pathology
Volume 58, Issue 4
January 2024
Pages 281-300

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