Document Type : Research Article
Authors
1
Department of Plant Protection, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
2
prof. of Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, AREEO, BandarAbbas, Iran
Abstract
The present study aimed to evaluate the resistance of 14 common tomato cultivars in the south of Kerman
province and Hormozgan province, including Afra, Badro, Basimo, Bernetta, Brivio, Canyon, Comodoro,
Elisa, Golsar, Karun, Matin, Sunseed 6189, 4129 and 8320 at the stage of four true-leaves against Fusarium
oxysporum f. sp. radicis-lycopersici, under greenhouse conditions and to study the presence or absence of frl
resistance gene in the tomato cultivars based on the SCARFrl marker. Based on the results of comparison of
the disease index, morphological characteristics including root length, shoot height, root fresh and dry
weight, shoot fresh weight and total fresh weight as well as frl gene amplification using SCARFrl-F/SCARFrlR primers, Canyon, Comodoro, Karun and 4129 were recognized as the most resistant and Bernetta, Elisa,
Golsar and Matin were recognized as the most sensitive cultivars to FORL UJFCC1919. Other cultivars
including Afra, Badro, Basimo, Brivio, Sunseed 6189 and 8320 showed different degrees of sensitivity to
this pathogen. Using SCARFrl-F/SCARFrl-R primers, a 1000 bp fragment was amplified in the cultivars
Canyon, Comodoro, Karun and 4129 which indicates the presence of frl gene with dominant homozygous
(RR) alleles. Using this primer, a 950 bp fragment was amplified in Bernetta, Elisa, Golsar, Matin and 8320
cultivars, which indicates the absence of the frl gene and the presence of homozygous recessive (rr) alleles.
In addition, in Afra, Badro, Basimo, Brivio and Sunseed 6189 cultivars, two 900-1000 bp fragments were
amplified, which indicates the existence of heterozygous (Rr) alleles in these cultivars.
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