Document Type : Research Article
Authors
1
, Department of Plant protection, School of Agriculture, Shiraz University, Iran
2
Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran
Abstract
So far, several bacterial diseases, including wheat stripe spots caused by Xanthomonas translucens and mild
bacterial mosaics caused byClavibacter tessellarius , have been reported in Poaceae family plants. In recent
years, reports have been published on the presence of Clavibacter zhangzhiyongii bacteria in barley plants in
some countries, such as Australia and China. This research was conducted to track and identify the
mentioned bacteria using biochemical and molecular methods and determining its host range. For this
purpose, in the years 2021 and 2022, wheat, barley, and weeds with symptoms of brown spots, mild mosaic,
decay, and yellowing of leaves were sampled in the provinces of Golestan, Bushehr, Qazvin, Zanjan, and
Fars. Bacterial isolation was performed according to standard bacteriological methods. Phenotypic and
biochemical tests identified isolates and housekeeping genes atpD, gyrB, ppK, and recA. Pathogenicity tests
were performed by spraying and stem injection. Based on biochemical and molecular tests, 15 isolates of the
genus Clavibacter were identified, and among the studied isolates, three isolates were identified as
C. zhangzhiyongii.
Keywords
)