Analysis of the expression pattern of NH1, thionine, and lipoxygenase in response to the rice bacterial blight disease caused by Xanthomonas oryzae pv. oryzae

Document Type : Research Article

Authors

1 Department of Plant Protection, Faculty of Crop Sciences. Sari Agricultural Sciences and Natural Resource University

2 Industrial Fungi Biotechnology Department of RIIB. ACECR.

3 Department of Plant Protection, Faculty of Agriculture,. University of Zabol

4 Department of Plant Protection, University of Zabol

5 Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources

Abstract

Bacterial blight caused by Xanthomonas oryzae pv. oryzae is one of the most destructive rice diseases in most regions. Understanding the biochemical and molecular interaction plays an important role in designing a specific strategy for the improvement and resistance of plants as the most effective and economical management method. In the interaction between the pathogen and the host, hundreds of genes are regulated and expressed, and in most cases, the difference between resistance and sensitivity is related to time and the amount of these changes compared to the difference in the expression of a set of genes. This study evaluated the expression pattern of NH1, thionine, and lipoxygenase genes at different intervals after inoculation in resistant (Khazar) and susceptible (local Tarom) cultivars to bacterial blight using qRT-PCR. The study of the expression pattern of the investigated genes showed that the trend of increasing their expression in the first period after inoculation (12 hours) in the resistant variety Khazar was significantly different from the susceptible variety. Despite the decrease in the expression of these genes after the initial hours, the gene expression ratio in the two susceptible and resistant varieties was significantly different at the 5% level up to 72 hours after inoculation. Increasing the expression of the mentioned genes and inducing various related systemic resistance pathways as a part of the defense mechanism of rice has played an important role in the resistance of the commercial cultivar Khazar to rice bacterial blight.

Keywords

References

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Iranian Journal of Plant Pathology
Volume 60, Issue 1
April 2025
Pages 33-47

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