The First Report of Alfalfa mosaic virus Occurrence on Pepper and Tomato Crops Based on Partial Nucleotide Sequence analysis of the Virus Coat Protein Open Reading Frame From Hormozgan Province, the Hub of Tomato Production in Iran

Document Type : Short Report

Authors

1 PhD Student of Plant Pathology, Faculty of Agriculture, Shiraz University

2 Professor, Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran.

3 Assistant professor of plant pathology, Department of Agriculture, Payame Noor University, Thehran, Iran.

Abstract

Emerging plant viral diseases account for serious threats to agricultural crop yields and food security in many parts of the world. in spring 2023 revealedsevere leaf chlorotic spots, yellowing symptoms, and fruit necrosis in tomato (Haji-Abad) and pepper (Bandar-Abbas) plants (Figure 1). To confirm AMV infections, total RNA was extracted from five symptomatic and one asymptomatic samples of tomato and pepper plants using TRIzol reagent (Sinaclone), followed by reverse transcription-polymerase chain reaction (RT-PCR) assays with a set of specific AMV primers (AMV-R, AMV-F) targeting a 780-nucleotide segment of the coat protein gene (Masoumi et al., 2012). PCR products of the expected size (approximately 780 bp) were obtained from all of the symptomatic samples (five samples of tomato and pepper plants), while no amplified products were generated from any of the healthy leaf samples of tomato and pepper plants. and sequenced directly using a paired-end sequencing strategy (Sinohe Co. Iran). Multiple alignments of the obtained nucleotide sequences (corresponding to a part of CP gene) with 15 AMV isolates obtained from GenBank showed the highest identity (97.9%. for IR-To-01 isolate and 98.3% for IR-Pep-03 isolate) with an Italian AMV strain See-1 isolated from Sechium elude and the lowest identity (95.8% for IR-To-01 and 95.4% for IR-Pep-03) with an Iranian AMV isolate (IRWS2) from Wisteria sinensis. Phylogenetic analysis grouped Iranian isolates with European strains, while, the other AMV isolates (including Iranian, Asian and American isolates) were grouped into a separate clade (Figure 2), suggesting a possible introduction through seed trade.

Keywords

References

Bananej, K., Mosahebi, G., & Okhovat, M. (1995). Identification of alfalfa mosaic (AMV), cucumber mosaic (CMV), bean common mosaic (BCMV) and bean yellow mosaic (BYMV) viruses in alfalfa fields in Karadj, Iran. In Proceedings of the 12th Iranian Plant protection Congress 2 7 September 1995 Karadj.
Bol, J. F. (1999). Alfalfa mosaic virus and ilarviruses: involvement of coat protein in multiple steps of the replication cycle. Journal of General Virology, 80(5), 1089-1102. https://doi.org/10.1099/0022-1317-80-5-1089.
Deng, D., McGrath, P. F., Robinson, D. J., & Harrison, B. D. (1994). Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of applied Biology, 125(2), 327-336. https://doi.org/10.1111/j.1744-7348.1994.tb04973.x.
Esfandiari, N., Mosahebi, G. H., & Mozafari, J. (2005). Detection of Alfalfa mosaic virus (AMV) in pea field in Iran. Communications in agricultural and applied biological sciences, 70(3), 407-410. PMID: 16637206 .
Gibbs, A., & Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16. https://doi.org/10.1016/S0166-0934(96)02103-9.
Golnaraghi, A. R., Shahraeen, N., Pourrahim, R., Farzadfar, S., & Ghasemi, A. (2004). Occurrence and relative incidence of viruses infecting soybeans in Iran. Plant Disease, 88(10), 1069-1074.
Hassanalilou, S., Pourrahim, R., Bakhshi Khanik, G. R., & Farzadfar, S. (2010). Detection of Three Viruses Causing Mosaic Diseases in Alfalfa Fields of West Azarbaijan Province. Proc. 19th Iranian Plant Protectin Congrres.
Li, Y., Tan, G., Lan, P., Zhang, A., Liu, Y., Li, R., & Li, F. (2018). Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers. Journal of Virological Methods, 259, 122-128. https://doi.org/10.1016/j.jviromet.2018.06.012.
Mangoli, F., Massumi, H., Alipour, F., Maddahian, M., Heydarnejad, J., Hosseinipour, A., ... & Varsani, A. (2019). Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates. Journal of Plant Pathology, 101, 735-742. https://doi.org/10.1007/s42161-019-00275-w.
Mangoli, F., Massomi, H., Heydarnejad, J., & Poor, A. H. (2012). Host range and partial molecular characterization of Alfalfa mosaic virus isolates in Kerman province. CABI Record Number: 20133085972.
Masoumi, H., Maddahian, M., Heydarnejad, J., Hoseinpour, A., & Farahmand, A. (2012). Incidence of viruses infecting alfalfa in the southeast and central regions of Iran. Journal of Agriculture Science and Technology, 14: 1141-1148. http://dorl.net/dor/20.1001.1.16807073.2012.14.5.5.3.
Massumi, H., Maddahian, M., Heydarnejad, J., Pour, A. H., & Farahmand, A. (2012). Incidence of viruses infecting alfalfa in the southeast and central regions of Iran. ISSN (Electronic): 2345-3737.
Masoumi, H., & Hosseini, P. A. (2007). Serological characterization of Alfalfa mosaic virus in alfalfa (Medicago sativa) in some regions of Iran.
Masoumi, H., Jones, P., & Hague, N. (2005a). Antigenic Analysis of the Coat Protein of Alfalfa mosaic virus and its Involvement in Aphid Transmission.
Massumi, H., Jones, P., & Hague, N. (2005b). Partial epitope mapping of Alfalfa mosaic virus and the effect of coat protein gene mutation on aphid transmission. Iranian Journal of Biotechnology, 3(1), 31-40.
Rojas, M. R., Gilbertson, R. L., Russell, D. R., & Maxwell, D. P. (1993). Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease, 77(4), 340-347. http://dx.doi.org/10.1094/PD-77-0340.
Zeynaddini, A., Jafarpour, B., & Rastegar, M. F. (2005). Study of Alfalfa mosaic virus in central and northern regions of Khorasan province. JWSS-Isfahan University of Technology, 9(2), 147-157. http://dorl.net/dor/20.1001.1.24763594.1384.9.2.13.3.
Iranian Journal of Plant Pathology
Volume 59, Issue 1
May 2024
Pages 64-68

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