Assessment Resistance Strains of Pseudomonas syringae pv. syringae from Different Plant Hosts to Copper Compounds

Document Type : Research Article

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Abstract

Strains of Pseudomonas syringae pv. syringae (Pss) are pathogenic in various types of plants, sometimes causing economic damage that requires chemical control. In Chaharmahal va Bakhtiary province, this pathogen damages stone fruit trees, wheat, beans, and greenhouse plants like cucumber. This study investigated the resistance of 62 Pss strains from different hosts to copper ions (copper sulfate) in a culture medium. Results showed that 37.1% and 32.3% of the strains were resistant to 100 and 200 µg/ml copper sulfate concentrations, respectively. Five copper-resistant strains from peach, almond, cherry, wheat, and beans and one sensitive strain from cucumber, were selected for further evaluation. The effect of various formulations containing copper compounds on inhibiting the growth of these strains in the culture medium was assessed. All five isolates showed varying resistance to copper compounds (copper oxychloride, copper oxide, copper carboxylate, and Bordeaux mixture). Doubling the concentration of these compounds did not significantly affect bacterial growth. However, adding mancozeb, zinc sulfate, or iron sulfate to each copper compound significantly inhibited bacterial growth on the culture medium. The copper carboxylate formulation combined with mancozeb was the most effective inhibition. The CopA gene, responsible for copper resistance, was detected in the five resistant strains and other Pss strains, confirming the results of the experiments. There is resistance to copper compounds in various Pss strains. compounds with a synergistic effect added to copper compounds. The most influential formulation identified in this study is a combination of copper carboxylate or copper oxide with mancozeb and urea.

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References

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Iranian Journal of Plant Pathology
Volume 59, Issue 2
July 2024
Pages 69-84

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