Evaluation of resistance of different tomato cultivars against Fusarium oxysporum f. sp. radicis-lycopersici and molecular identification of resistant cultivars based on SCARFrl marker in the south of Kerman Province and Hormozgan Province

Document Type : Research Article

Authors

1 Department of Plant Protection, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

2 Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, AREEO, Bandar Abbas, Iran

Abstract

The present study aimed to evaluate the resistance of 14 common tomato cultivars in the south of Kerman province and Hormozgan province, including Afra, Badro, Basimo, Bernetta, Brivio, Canyon, Comodoro, Elisa, Golsar, Karun, Matin, Sunseed 6189, 4129 and 8320 at the stage of four true-leaves against Fusarium oxysporum f. sp. radicis-lycopersici, under greenhouse conditions and to study the presence or absence of frl resistance gene in the tomato cultivars based on the SCARFrl marker. Based on the results of comparison of the disease index, morphological characteristics including root length, shoot height, root fresh and dry weight, shoot fresh weight and total fresh weight as well as frl gene amplification using SCARFrl-F/SCARFrl-R primers, Canyon, Comodoro, Karun and 4129 were recognized as the most resistant and Bernetta, Elisa, Golsar and Matin were recognized as the most sensitive cultivars to FORL UJFCC1919. Other cultivars including Afra, Badro, Basimo, Brivio, Sunseed 6189 and 8320 showed different degrees of sensitivity to this pathogen. Using SCARFrl-F/SCARFrl-R primers, a 1000 bp fragment was amplified in the cultivars Canyon, Comodoro, Karun and 4129 which indicates the presence of frl gene with dominant homozygous (RR) alleles. Using this primer, a 950 bp fragment was amplified in Bernetta, Elisa, Golsar, Matin and 8320 cultivars, which indicates the absence of the frl gene and the presence of homozygous recessive (rr) alleles. In addition, in Afra, Badro, Basimo, Brivio and Sunseed 6189 cultivars, two 900-1000 bp fragments were amplified, which indicates the existence of heterozygous (Rr) alleles in these cultivars.

Keywords

References

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Iranian Journal of Plant Pathology
Volume 59, Issue 2
July 2024
Pages 116-132

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