First report of a ‘Candidatus phytoplasma asteris’ related (16SrI-B) phytoplasma associated with Rosa canina yellows disease in Iran

Document Type : Short Report

Authors

1 -

2 Assistant Professor of Plant Protection Research Department, Yazd Agricultural And Natural Resources Research And Education Centre, Yazd, Iran.

3 Plant Protection Research Department Fars Agricultural and Natural Resources Research and Education Center, Zarghan, Iran

4 Fars

Abstract

In a 2022 survey conducted in Jahrom, Fars province, Iran, the dog rose yellows (DRY) disease was observed in Rosa canina L. plants. The disease manifested as severe yellowing, leaf curling, reduced flowering, abnormal flowers and stunted growth (Fig. 1). DNA from 15 symptomatic and four asymptomatic plants was tested for phytoplasma presence. Nested PCR with P1/P7 and R16F2n/R16R2 primers (Lee et al., 1998) yielded 1.25 kbp fragments exclusively from symptomatic plants. The sequences from these samples were identical and one was submitted to GenBank (Accession No. OQ332406). BLAST analysis revealed that the Jahrom DRY (JDRY) phytoplasma sequence matched with two Iranian 16SrI-B strains: Bajgah Tragopogon dubius phyllody (KR262955) and Zarghan onion yellows (MG519854). Phylogenetic analysis placed JDRY phytoplasma within the 16SrI group, closet to the 16SrI-B subgroup (Fig. 2). This report marks the first association of a 16SrI-B phytoplasma with DRY disease in Iran. The leafhopper Circulifer haematoceps, known to vector similar phytoplasma associated with rapeseed phyllody (Salehi et al., 2011), is yet to be tested for its ability to transmit Rosa canina yellows phytoplasma.

Keywords

References

Esmaeilzadeh-Hosseini, S.A., Azadvar, M., Babaei, G., Salehi, M., Bertaccini, A. 2023. Diversity, distribution and status of phytoplasma diseases in Iran. In: Phytoplasma Diseases in Asian countries. Vol. 1: Diversity, Distribution and Current Status. Eds. Tiwari, A.K., Caglayan, K., Al-Sadi, A., Azadvar, M., Abeysinghe, S. Academic Press. 368p.
 Lee, I.-M., Gundersen-Rindal, D.E., Davis, R.E., Bartoszyk, I.M. 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology 48: 1153-1169.
Salehi, M., Izadpanah, K., Siampour, M. 2011. Occurrence, molecular characterization and vector transmission of a phytoplasma associated with rapeseed phyllody in Iran. Journal of Phytopathology 159: 100-105
Iranian Journal of Plant Pathology
Volume 59, Issue 2
July 2024
Pages 133-137

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