Document Type : Research Article
Authors
1
Department of Plant Protection, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari
2
Department of Plant Protection. Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3
Department Plant products Faculty of Agriculture and Natural resources, Gonbad Kavous University, Gonbad Kavous, Iran
4
Department of Plant Protection. Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Ira
5
Department of Agronomy, Islamic Azad University, Gorgan Branch, Gorgan, Iran
Abstract
Soybean charcoal rot, caused by Macrophomina phaseolina, is an economically important disease affecting various oilseed crops in northern regions, particularly in Golestan and Mazandaran provinces. This fungus can lead to seedling death, seedling blight, crown rot, stem rot, charcoal rot, and root rot under favorable conditions. The current study utilized a factorial design in a completely randomized setup with 3 replications and 2 factors. The first factor included T. harzianum and M. phaseolina, while the second factor involved different sampling times. Sampling was conducted at 24, 48, 72, and 96 hours post-pathogen inoculation to assess the activities of CAT, PPO, and GPX enzymes. Disease progression was evaluated 31 days after inoculation. The study found that the activity of CAT, PPO, and GPX enzymes in treated soybeans was higher during the initial hours compared to the control group. Additionally, the severity of M. phaseolina disease was lower in the T. harzianum treatment group than in the positive control. These findings contribute to a better understanding of the interactions between T. harzianum and the biological control of M. phaseolina, demonstrating its efficacy in disease management.
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