Effect of temperature, life stage and soil drainages of host and non-host plants on hatching of cabbage cyst nematode, Heterodera cruciferae

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Abstract

Stimulation of egg hatching of cabbage cyst nematode (Heterodera cruciferae) were investigated by soil drainages of host (kohlrabi and white cabbage) and non-host (lettuce) plants in different life stages, under different temperature conditions including 5, 10, 15, 20 and 25°C. Soil drainages of non-host plant and three and six-months-old seedlings of host plants were collected at 2, 4, 6 and 8 leaves developmental stages. Different concentrations of soil drainagesincluding undiluted soil drainages, soil drainagesdiluted 1:4 or 1:16 with water were used. Pot’s draining water and tap water were used as two negative controls and 0.3 M ZnCl2 solution as a positive control. Twenty-five cysts were stored in a 24-well plastic plate containing the soil drainagessolutions. The rate of eggs hatching was noted with a week intervals up to three weeks. The results showed that dilution of six-months-old white cabbage soil drainages1:4 at six leaves stage enhanced stimulation of hatching at 20°C compared with other treatments.
 

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