نوع مقاله : مقاله کوتاه پژوهشی
نویسندگان
1 مسئول مکاتبه
2 نویسنده
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Ceratorhizahydrophila is frequently isolated from rice sheath spot in paddy rice of Guilan province. In this study, morphology, pathogenicity and its phylogenetic affinities based on rDNA sequence data were studied. Results revealed that the fungus can infect rice plants and cause small yellow to pale brown spots on the leaf sheaths one week after the inoculation. On PDA colonies were white at the beginning and were eventually changed to brown, with visible growing rings. Sclerotia were usually round, regular, very small, measured 275-737.5 µm, and were formed on the surface of Petri dishes four days after the cultivation. They were initially white, and then changed to buff or blackish brown. Mycelial cells were 3.75-7.5 × 37.5-192.5 µm. To study phylogenetic position and molecular identification, the 530 bp of rDNA ITS region was sequenced. Results showed that C. hydrophila is closely related to Rhizoctonia species rather than other Sclerotium taxa. These results are consistent with the morphology of colony and sclerotia of this fungus. This is the first report of the pathogenecity of this fungus on rice from Iran.
کلیدواژهها [English]
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