تاکسونومی، فیلوژنی و بیماری‌زایی گونه‌های Pythium در شالیزارهای برنج استان فارس

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دانش آموخته دانشگاه شیراز

2 عضو هیئت علمی/دانشگاه شیراز

چکیده

به ­منظور بررسی گونه­های پیتیومی شالیزارهای برنج استان فارس طی سال­های 1392 تا 1394 از خاک، آب آبیاری، ریشه و طوقه­ی گیاهان بیمار نمونه­برداری انجام گرفت. بر اساس نتایج حاصل ازمطالعات ریخت­شناختی، ریخت­سنجی و فیزیولوژیکی جدایه­ها به همراه بررسی­های فیلوژنتیکی فاصله­ی ترانویسی­شده­ی داخلی (آی­تی­اِس) دی­اِن­اِی ریبوزومی با روش پیوست همسایه­ها، 14 گونه­ی Pythium شامل Py. aphanidermatum، Py. catenulatum، Py. coloratum، Py. debaryanum، Py. dissotocum، Py. hydnosporum، Py. inflatum، Py. kashmirense، Py. nunn، Py. oopapillum، Py. plurisporium، Py. porphyrae، Py. pyrilobum و Py. rhizo-oryzae شناسایی شد. تمامی گونه­های یاد شده، به جز گونه­های Py. dissotocum، Py. inflatum و Py. rhizo-oryzae، برای نخستین­بار از فراریشه­ی برنج در دنیا گزارش می­شوند. گونه­های Py. oopapillum، Py. plurisporium، Py. porphyrae، Py. rhizo-oryzae برای ایران جدیدند. فراوان­ترین گونه­های جداشده Py. aphanidermatum، Py. rhizo-oryzae و Py. catenulatum بودند. بیماری­زایی گونه­های Py. debaryanum، Py. oopapillum، Py. plurisporium، Py. porphyrae و Py. rhizo-oryzae روی برنج برای نخستین­بار در دنیا گزارش می­شود.

کلیدواژه‌ها

عنوان مقاله [English]

Taxonomy, Phylogeny and Pathogenicity of Pythium Species in Rice Paddy Fields of Fars Province

نویسندگان [English]

  • F. Salmaninezhad 1
  • Reza Mostowfizadeh-Ghalamfarsa 2
1 Shiraz University
2 Shiraz University
چکیده [English]

In order to investigate the Pythium species of the rice paddy fields of Fars Province, Iran during 2013 to 2015, infected roots and crowns together with soil around seedlings and irrigation water were sampled. According to the morphological, morphometrical and physiological studies along with phylogenetic analyses based on internal transcribed spacer (ITS) of ribosomal DNA, fourteen Pythium species including Py. aphanidermatum, Py. catenulatum, Py. coloratum, Py. debaryanum, Py. dissotocum, Py. hydnosporum, Py. inflatum, Py. kashmirense, Py. nunn, Py. oopapillum, Py. plurisporium, Py. porphyrae, Py. pyrilobum, and Py. rhizo-oryzae were identified. All species are reported for the first time in the world from rice rhizosphere except Py. dissotocum, Py. inflatum and Py. rhizo-oryzae. Pythium oopapillum, Py. plurisporium, Py. porphyrae and Py. rhizo-oryzae were new to Iran. Pythium aphanidermatum, Py. rhizo-oryzae and Py. catenulatum were the most abundant species. Pathogenicity of Py. debaryanum, Py. oopapillum, Py. porphyrae, Py. plurisporium and Py. rhizo-oryzae on rice plants were reported in this study for the first time, worldwide.

کلیدواژه‌ها [English]

  • identification
  • Oomycota
  • Pythiaceae
  • root rot

مراجع

 
Abad G., Shew H.D., Grand L.F. and Lucas L.T. 1995. A new species of Pythium producing multiple oospores isolated from bentgrass in North Carolina. Mycologia87:896-901.
Ahn S.W., Bonman J.M., Brandon D.M., Groth D.E., Gunnell P.S., Hibino H., Hollier C.A., Lee F.N., Mew T.W., Prot J.C., Rush M.C., Schneider R.W., Webster R.K., and Whitney G. 1992. Compendium of Rice Diseases. St. Paul. Min., USA: APS Press. 86 p.
Al-Sheikh H. 2010. Two pathogenic species of Pythium: P. aphanidermatum and P. diclinum from a wheat field. Saudi Journal of Biological Science17: 347-352.
Ann P.J., Huang J.H., Wang I.T. and Ko W.H. 2006. Pythiogeton zizaniae, a new species causing basal stalk rot of water bambo in Taiwan. Mycologia 98: 116-120.
Bala K., Robideau G.P., Désaulniers N., de Cock A.W. A. M. and Lévesque C. A. 2010a. Taxonomy, DNA barcoding and phylogeny of three new species of Pythium from Canada.Persoonia 25:22-31.
Bala K., Robideau G.P., Lèvesque A., de Cock A.W.A.M., Adad Z.G., Lodhi A.M., Shahzad S., Ghaffar A. and Coffey M.D. 2010. Phytopythium Adad, de Cock, Bala, Robideau, Lodhi and Lèvesque gen. nov. and Phytopythium sindhum Lodhi, Shahzad and Lèvesque sp. nov. Persoonia24:136-137.
Banihashemi Z., MacDonald J.D. and Stite J. 1992. Combine baiting and ELISA to detect and quantify Phytophthora spp. in container media. Phytopathology82:1101(Abstr.).
Banihashemi Z. 1989. Study of pistachio gummosis in southern provinces of Iran. Proceedings of the 9th Iranian Plant Protection Congress,Mashhad, Iran, p.87.
Banihashemi Z. and Fatehi J. 1989. Reaction of cucurbit cultivars to Phytophthora drechsleri and P. capsici in greenhouse. Proceeding of the 9th Iranian Plant Protection Congress, Mashhad, Iran, p.89.
Bolboli Z. and Mostowfizadeh-Ghalamfarsa R. 2015. Phylogenetic relationships and taxonomic characteristics of Pythium spp. isolates in cereal fields of Fars Province. Iranian Journal of Plant Pathology 51: 471-492.
 Bridge J. and Page S.L.J. 1982. The rice root-knot nematode, Meloidogyne graminicola, on deep water rice (Oryza sativa subsp. indica). Revue de Nématologie5:225-232.
Cooke D‌.E.L. and Duncan J.‌M. 1997. Phylogenetic analysis of Phytophthora species based on the ITS1 and ITS2 sequences of ribosomal DNA. Mycological Research 101:667-677.
Dick M.W. 1990. Keys to Pythium. University of Reading, UK. 64 p.
Elad Y., Lifshitz R. and Baker R. 1985. Enzymatic activity of the mycoparasite Pythium nunn during interaction with host and non-host fungi. Physiological Plant Pathology27:131-148.
Erwin D.C. and Ribeiro O.K. 1996. Phytophthora diseases worldwide. APS Press, St. Paul, Minnesota, USA. 592 p.
Fisher A.J. 1996. Integrated red rice management in Latin American rice fields. Proceedings of the 2nd International Weed Control Congress Copenhagen, Denmark, p.653.
Fukutomi M., Akai S. and Shiraishi M. 1971. Fine structure of anthridia and oogonia of Phytophthora macrospora, the downy mildew fungus of rice plants. Mycopathologia et Mycologia Applicata 43: 249-258.
Furuya H., Matsumoto T., Fuji S. and Naito H. 2003. Inconspicuous restraint of rice seedling growth by root-infecting fungi in soil of a rice paddy field. Journal of General Plant Patholoty 69: 115-119.
Furuya H., Tubaki K., Matsumoto T., Fuji S. and Naito H. 2005. A case study of infection of rice roots by deleterious fungi and minor pathogenic Pythium species in a paddy field. Journal of General Plant Pathology 73: 8-14.
Furuya H., Matsumoto T., Fuji S. and Naito H. 2007. A case study of infection of rice roots by deleterious fungi and minor pathogenic Pythium species in a paddy field. Journal of General Plant Pathology 73: 8-14.
Gunnell P.S. and Webster R.K. 1988. Crown and root rot of cultivated wild rice in California caused by Phytophthora erythroseptica sensu lato. Plant Disease 72:909-910.
Hall T‌.A. 1999. Bio Edit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium 41:95-98.
Hsieh H.J. 1978. An annotated list of Pythium in Taiwan. Botanical Bulletin Academia Sinica 19:199-205.
Huang J.H., Chen C.Y., Lin Y.S., Ann P.J., Huang H.C. and Chung W.H. 2013. Six new species of Pythiogeton in Taiwan, with an account of the molecular phylogeny of this genus.Mycoscience 54: 130-147.
Lenney J.F. and Klemmer H.W. 1966. Factors controlling sexual reproduction and growth in Pythium graminicola. Nature 209: 1365-1366.Jeffers S.N. and Martin S.B. 1968. Comparision of two media selective for Phytophthora and Pythium species. Plant Disease70:1035-1043.
Kato M., Minamida K., Tojo M., Kokuryu T., Hamaguchi H. and Shimada S. 2013. Association of Pythium and Phytophthora with pre-emergence seedling damping-off of soybean grown in a field converted from a paddy field in Japan. Plant Production Science 16: 95-104.
Kobayashi S., Uzuhashi S., Tojo M. and Kakishima M. 2010. Characterization of Pythium nunn newly recorded in Japan and its antagonistic activity against P. ultimum var. ultimum. Journal of General Plant Pathology 76:278-283.
Lévesque C.A. and de Cock A.W.A.M. 2004. Molecular phylogeny and taxonomy of the genus Pythium. Mycological Research 108:1363-1383.
Mew T.W. 1991. Rice diseases. pp. 187-236. In: B.S. Luh (Ed), Rice. Springer Science +Business media, New York, USA.
Mirsoleimani Z. and Mostowfizadeh-Ghalamfarsa R. 2013. Characterization of Phytophthora pistaciae, the casual agent of pistachio gummosis, based on host range, morphology and ribosomal genome. Phytopathologia Mediterranea53:501-506.
Mostowfizadeh-Ghalamfarsa R. and Banihashemi Z. 2005. Identification of soil Pythium species in Fars Province of Iran. Iranian Journal of Science and Technology 29:79-87.
Mousanejad S., Momeni A., Khosravi V. and Javannikkhah M. 2009. Identification of the physiological races of the blast causal agent in Guilan and Mazandaran Provinces. Journal of Plant Diseases3:257-262.
Padashtdehkahi F., Akhavat S.M., Nikkhah M.J. and Mahmoudi S.B. 2009. Optimization of inoculation method of sheath blight in order to separate the actual reaction of rice cultivars in farm. Iranian Journal of Plant Protection Science 40:99-108.
Paulitz T.C. and Baker R. 1987. Interactions between Pythium nunn and Pythium ultimum on bean leaves. Canadian Journal of Microbiology 34:947-951.
Paulitz T.C. and Baker R. 1988. The formation of secondary sporangia by Pythium ultimum: the influence of organic amendments and Pythium nunn. Soil Biology and Biochemistry 20:151-156.
Paulitz T.C., Ahmed J. S. and Baker R. 1990. Integration of Pythium nunn and Trichoderma harzianum isolate T-95 for biological control of Pythium damping-off of cucumber. Plant and Soil 121:243-250.
Robideau G.P., de Cock A.W.A.M., Coffey M.D., Volgmayr H., Brouwer H., Bala K., Chitty D.W., Desaulniers N., Eggertson Q.A., Gachon C.M., Hu C.H., Kupper F.C., Rintoul T.L., Sarhan E., Verstappen E. C., Zhang Y., Bonants P. J., Ristaino J. B. and  Lévesque A. C. 2011. DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer. Molecular Ecology Resources 11:1002-1011.
Safari Motlagh M.R., Javadzadeh Haghighat S.A., Rashidi V. and Yaghoubi B. 2011. Reaction of some rice cultivars against the fungus Curvularia lunata in Gilan Province. Journal of Biological Sciences5: 59-66.
Silue D., Nottegham J. L. and Tharreau D. 1991. Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem. Phytopathology 82:577-580.
Strimmer K. and von Haeseler A. 1996. Quartet puzzling - A quartet maximum likelihood method for constructing tree topologies. Molecular Biology and Evolution 13:964-969.
Tan K.H. 1996. Soil sampling, preparation and analysis. Marcel Dekker Inc., New York, USA. 639 p.
Tavakoli F., Aghajani M. A., Etebarian H. R. and Rostami M. 2011. Evaluation of rice sheath blight disease in rice paddy fields of Mazandaran Province. Journal of Plant Disease Research 1:13-23.
Thompson J.‌D., Gibson T.‌J., Plewniak F., Jeanmougin F. and Higgins D.G. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25:4876-4882.
Thompson R.A., Quisenberry S.S., Robinson J.F. and Schneider R.W. 1991. Effect of rice water weevil (Coleoptera: Curculionidae) and Pythium spp. on water-seeded rice. Journal of Economic Entomology 84: 317-323.
Tuit J. 1969. Plant pathological methods. Burgess Publishing Co., USA.
van Buyten E. and Höfte M. 2013. Pythium species from rice roots differ in virulance, host colonization and nutritional profile. BMC Plant Biology 13:1-17.
van der Plaäts-Niterink A.‌J. 1981. Monograph of the genus Pythium. Studies in Mycology. No. 21. Centraalbureau voor Schimmelcultures, The Netherlands. 198 p.
Ward D.E. and Shipton W.A. 1984. Root rot of papaw caused by Pythium pyrilobum. Australasian Plant Pathology 13:25-27.
Webster R.K. and Gunnell P. S. 1992. Compendium of rice ciseases. APS Press, USA. 86 pp.
Webster R.K., Hall D.H., Heeres J., Wick C.M. and Brandon D.M. 1970. Achlya klebsiana and Pythium species as primary causes of seed rot and seedling disease of rice in California. Phytopathology 60:964-968.
White T.‌J., Bruns T., Lee S. and Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In: M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (eds), PCR protocols: A guide to methods and applications. Academic Press, San Diego, USA.
Zhang W. and Watson A.K. 2000. Isolation and partial characterization of phytotoxins produce by Exserohilum monoceras, a potential bioherbicide for control of Echinochola species. Proceedings of the 10th International Symposium on Biocontrol. Bozman, Montana, p. 125.
Zhang W.M. Mood K. and Watson A.K. 1996. Responses of Echinochola species and rice (Oryza sativa) to indigenous pathogenic fungi. Plant Disease 80:1053-1058

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