تاثیر سویه‌های غیربیماری‌زای Pseudomonas روی شدت بیـماری ایجاد شده توسط بیمارگرهای Botrytis cinerea وalternata Alternaria در گیاه گوجه‌فرنگی و آرابیدوپسیس

نوع مقاله : مقاله کامل پژوهشی


1 ﺑﺨﺶ گیاهپزشکی، دانشکده علوم کشاورزی، ﺩﺍﻧﺸﮕﺎﻩ گیلان، رشت.

2 ﺑﺨﺶ گیاهپزشکی ، دانشکده کشاورزی، ﺩﺍﻧﺸﮕﺎﻩ شیراز، شیراز.


سودوموناس­های غیربیماری­زا از مهمترین عوامل کنترل­زیستی می­باشند، که در مدیریت بیماری­های گیاهی استفاده می‏شوند. در این تحقیق اثر چهار سویه از (Pf1, Pf2, Pf3, Pf4)Pseudomonas fluorescens و یک سویه از Pseudomonas putida (P13) روی بیماری برگی ناشی از Botrytis cinerea و  Alternaria alternataسنجیده شد. هدف این تحقیق بررسی توانایی سودوموناس­های فلورسنت در کاهش شدت بیماری در گیاه گوجه‏فرنگی (ارقام کوئین و مجار) و آرابیدوپسیس بود. بررسی تاثیر عوامل کنترل زیستی در کاهش غیرمستقیم شدت بیماری­های فوق نشان داد، که بیشترین کاهش بیماری مربوط به سویه­های Pf3 و سویه­ی P13 بود، که سویه Pf3 قادر به کاهش میانگین قطر لکه تا حدود 25 درصد و سویه P13 نیز قادر به کاهش میانگین قطر لکه تا حدود 30 درصد نسبت به شاهد بودند. بررسی توانایی عوامل کنترل زیستی در کنترل مستقیم بیمارگر نشان داد، که سویه­های به‌کار برده شده تفاوت معنی‏داری در کنترل بیمارگر داشتند و سویه P13 بیشترین توانایی را در کنترل بیمارگر داشت، به‏طوری‏که قادر بود تا حدود 45 درصد بیماری را نسبت به شاهد کاهش دهد. در نهایت سویه‏های P. fluorescen s (Pf3) و P. putida (P13) به عنوان سویه‏های قوی­تر معرفی شدند. این سویه‏ها علاوه برکاهش شدت بیماری در گیاه، توانایی افزایش رشد گیاه را نیز داشتند.


عنوان مقاله [English]

Effect of non-pathogenic Pseudomonas strains on the severity of diseases caused by Botrytis cinerea and Alternaria alternata on tomato and Arabidopsis plants

نویسندگان [English]

  • H. Raeisi 1
  • S.M. Taghavi 2
  • H. Hamzehzarghani 2
  • M. Ansari 2
  • M. Djavaheri 2
چکیده [English]

Non-pathogenic Pseudomonadsare the main biocontrol agents that are used in plant disease management. In this study, effects of four strains of Pseudomonas fluorescens (Pf1, Pf2, Pf3, Pf4), and P. putida (P13) were studied for their ability to elicit induced systemic resistance against diseases caused by Botrytis cinerea and Alternaria alternata on tomato (Super Majar and Queen cvs) and Arabidopsis. The ability of biocontrol agents to colonize roots of tomato cultivars and Arabidopsis and induce resistance was shown to be highest for P13, and Pf3 strains and P13, and Pf3 were able to reduce the average lesion diameter of diseased leaves to 30% and 25%, compared to that of control, respectively. The ability of biocontrol agents for direct control the pathogens was also evaluated. The results revealed that P. putida (P13) was the best biocontrol agent as measured by the reduction in disease severity. P13 reduced the average lesion diameter of diseased tomato leaves by about 45%. Finally, Pf3 and P13 were identified as more powerful strains. Moreover, these strains could induce some level of resistance against various pathogens and they had the ability to enhance plant growth.

کلیدواژه‌ها [English]

  • Biological control
  • Pseudomonas fluorescens
  • Pseudomonas putida
  • leaf pathogens
Achuo E.A., Audenaert K., Meziane, H. and Höfte M. 2004. The salicylic acid-dependent defence pathway is effective against different pathogens in tomato and tobacco. Plant Pathology 53: 65–72.
Audenaert K., De Meyer G. and Höfte M. 2002a. Abscisic acid determines basal susceptibility of tomato to Botrytis cinerea and suppresses salicylic acid-dependent signaling mechanisms. Plant Physiology 128: 491-501.
Audenaert K., Pattery T., Cornelis P. and Hofte M. 2002b. Induction of Systemic Resistance to Botrytis cinerea in Tomato by Pseudomonas aeruginosa 7NSK2:Role of Salicylic Acid, Pyochelin, and Pyocyanin. Molecular Plant-Microbe Interaction 15: 1147–1156.
Bakker P.A.H.M., Pieterse C.M.J. and Loon L.C.V. 2007. Induced systemic resistance by fluorescent Pseudomonas spp. Phytopathology 97: 239–243.
Barnett H.L. and Hunter B.B. 1998. Illustrated genera of imperfect fungi. 4th ed., APS Press, Saint Paul., Minnesota., USA.
Conrath U., Beckers G.J.M., Flors V., Garcı´a-Agustı´n P., Jakab G., Mauch F., Newman M.A., Pieterse C.M.J., Poinssot B., Pozo M.J., Pugin A., Schaffrath U., Ton J., Wendehenne D., Zimmerli L. and Mauch-Mani B. 2006. Priming: Getting ready for battle. Molecular Plant-Microbe Interaction 19: 1062–1071.
Conrath U. and Gollner K. 2008. Priming: it’s all the world to induced disease resistance. European Journal of Plant Pathology 121: 233–242.
Dimkpa C., Weinand T. and Asch F. 2009. Plant–rhizobacteria interactions alleviate abiotic stress conditions. Plant Cell Environment 32: 1682–1694.
Egamberdieva D., Lindstrom L. and La K. 2015. A synergistic interaction between salt-tolerant Pseudomonas and Mesorhizobium strains improves growth and symbiotic performance of liquorice (Glycyrrhiza uralensis Fish.) under salt stress. Applied Microbiology and Biotechnology 100: 2829–2841.
El Oirdi M., El Rahman T.A., Rigano L., El Hadrami A., Rodriguez M.C., Daayf F., Vojnov A. and Bouarab K. 2011. Botrytis cinerea manipulates the antagonistic effects between immune pathways to promote disease development in tomato. Plant Cell 23: 2405–2421.
Glick B.R. 2015. Biocontrol mechanisms. In: Glick BR (ed) Beneficial plant-bacterial interactions. Springer, Heidelberg. pp 123–157.
Gravel V, Antoun H. and Tweddell R.J. 2007. Growth stimulation and fruit yields improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: possible role of indole-acetic acid (IAA). Soil Biology and Biochemistry 39: 1968-1977.
Harman G.E., Howell C.R., Viterbo A., Chet I. and Lorito M. 2004. Trichoderma speciesopportunistic, avirulent plant symbionts. Nature Reviews Microbiology 2: 43–56.
Heidel A.J., Clarke J.D., Antonovics J. and Dong X. 2004. Fitness costs of mutations affecting the systemic acquired resistance pathway in Arabidopsis thaliana. Genetics 168: 2197–2206.
Iavicoli A., Boutet E., Buchala A. and Métraux J.P. 2003. Induced systemic resistance in Arabidopsis thaliana in response to root inoculation with Pseudomonas fluorescens CHA0. Molecular Plant-Microbe Interaction 16: 851–858.
Islam S., Akanda A.M., Prova A., Islam M.T. and Hossain M. 2015. Isolation and identification of plant growth promoting rhizobacteria from cucumber rhizosphere and their effect on plant growth promotion and disease suppression. Frontiers Microbiology 6: 1360.
Jha Y. and Subramanian R. 2014. PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity. Physiology and Molecular Biology of Plants 20: 201–207.
Kamilova F., Lamers G. and Lugtenberg B. 2008. Biocontrol strain Pseudomonas fluorescens WCS365 inhibits germination of Fusarium oxysporum spores in tomato root exudate as well as subsequent formation of new spores. Environment Microbiology 10: 2455–2461.
Kerepesi I. and Galiba G. 2000. Osmotic and salt-stress induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science 40: 482-487.
Majeed A., Abbasi M.K., Hameed S., Imran A. and Rahim N. 2015. Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers Microbiology 6: 198.
Meziane H., Van der Sluis I., Van Loon L.C., Höfte M. and Bakker P.A.H.M. 2005. Determinants of Pseudomonas putida WCS358 involvedin inducing systemic resistance in plants. Molecular Plant Pathology 6: 177-185.
Narayanan P., Parthasarathy S., Rajalakshmi J., Arunkumar K. and Vanitha S. 2016. Systemic elicitation of defense related enzymes suppressing Fusarium wilt in mulberry (Morus spp.). African Journal of Microbiology Research10: 813-819.
Pieterse C.M.J., Van Wees S.C.M., Hoffland E., Van Pelt J.A. and Van Loon L.C. 1996. Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression. Plant Cell 8: 1225–1237.
Pieterse C.M.J., Zamioudis C., Berendsen R.L., Weller D.M., Van Wees S.C.M. and Bakker P.A.H.M. 2014. Induced systemic resistance by beneficial microbes. Annual Review of Phytopathology 52: 347–375.
Planchamp C., Glauser G. and Mauch-Mani B. 2014. Root inoculation with Pseudomonas putida KT2440 induces transcriptional and metabolic changes and systemic resistance in maize plants. Frontiers Plant Science 5: 719.
Pozo M.J. and Azcon-Aguilar C. 2007. Unraveling mycorrhiza-induced resistance. Current Opinion in Plant Biology 10: 393–398.
Ryu C.M., Farag M.A., Hu C.H., Reddy M.S., Kloepper J.W. and Paré P.W. 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiology 134: 1017-1026.
Mirzaei S., Goltapeh E.M. and Shams-bakhsh M. 2007. Taxonomical studies on the genus Botrytis in Iran. Journal Agriculture Technology 3: 65-76.
Sarikhani M.R., Malboobi M.A., Aliasgharzad N., Greiner R. and Yakhchali B. 2010. Functional screening of phosphatase-encoding genes from bacterial sources. Iranian Journal of Biotechnology 8(4): 275-279.
Schaad N.W., Jones J.B. and Chum W. 2001. Laboratory guide for identfication of plant pathogenic bacteria. APS Press. 373 p.
Simmons E.G. 1992. Alternaria taxonomy: current status, view point, challenge. In Alternaria Biology, Plant Diseases and Metabolites (Schakowsky J. and Visconti A. eds). Elsevier Science Publishers. Amsterdam.
Ton J., Van Pelt J.A., Van Loon L.C. and Pieterse C.M.J. 2002. Differential effectiveness of salicylate-dependent and jasmonate/ethylene-dependent induced resistance in Arabidopsis. Molecular Plant-Microbe Interaction15: 27-34.
Van der Ent S., Van Wees S.C.M. and Pieterse C.M.J. 2009. Jasmonate signaling in plant interactions with resistance-inducing beneficial microbes. Phytochemistry 70:1581–1588.
Van Loon L.C., Bakker P.A.H.M. and Pieterse C.M.J. 1998. Systemic resistance induced by rhizosphere bacteria. Annual Review of Phytopathology 36: 453–483.
Van Loon L.C. and Bakker P.A.H.M. 2005. Induced systemic resistance as a mechanism of disease suppression by rhizobacteria. In Z. A. Siddiqui (Ed.), PGPR: Biocontrol and Biofertilization. pp 39–66.
Van Loon L.C. 2007. Plant responses to plant growth-promoting rhizobacteria. European Journal of Plant Pathology 119:243–254.
Van Wees S.C.M., Pieterse C.M.J., Trijssenaar A., Westende Y.A.M., Hartog F. and Van Loon L.C. 1997. Differential induction of systemic resistance in Arabidopsis by biocontrol bacteria. Molecular Plant-Microbe Interaction 10: 716–724.
Van Wees S.C.M., Van der Ent S. and Pieterse C.M.J. 2008. Plant immune responses triggered by beneficial microbes. Current Opinion in Plant Biology 11: 443–448.
Vanneste J.L., Cornish D.A., Spinelli F. and Yu J. 2004. Colonisation of apple and pear leavesby different strains of biological control agents of fire blight. Journal of the New Zealand Plant Protection 57: 49–53.
Verhagen B.W.M., Trotel-Aziz P., Couderchet M., Hofte M. and Aziz A. 2010. Pseudomonas spp.-induced systemic resistance to Botrytis cinerea is associated with induction and priming of defense responses in grapevine. Journal of Experimental Botany 61: 249–260.
Verhagen B., Trotel-Aziz P., Jeandet P., Baillieul F. and Aziz A. 2011. Improved resistance against Botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production. Phytopathology101(7):768–777.
Walker T.S., Bais H.P., Halligan K.M., Stermitz F.R. and Vivanco J.R. 2003. Metabolic profiling of root exudates of Arabidopsis thaliana. Journal of Agricultural and Food Chemistry 51: 2548–2554.
Walters D.R., Ratsep J. and Havis N.D. 2013. Controlling crop diseases using induced resistance: challenges for thefuture. Journal of Experimental Botany 64: 1263-1280.
Zahid M., Abbasi M.K., Hameed S. and Rahim N. 2015. Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.). Frontiers Microbiology 6: 207.
Zhang H., Xie X., Kim M.S., Kornyeyev D.A., Holaday S. and Paré P.W. 2008. Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta. Plant Journal 56: 264–273.