ردیابی سریع ویروس اس سیب‌زمینی با استفاده از نانوذرات طلای پوشش داده شده با آنتی‌بادی

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 هیات علمی

2 عضو هیات علمی

چکیده

علیرغم وجود روش­های مختلف در تشخیص ویروس­ها، زمان­بر بودن آزمون­ها یکی از مهمترین محدودیت­های آنها است. از این رو، توسعه روش­های سریع­تر برای صدور گواهی در ایستگاه­های قرنطینه و مدیریت ویروس­های گیاهی اهمیت شایانی دارد. در این تحقیق، با استفاده از فناوری نانو، نانوذرات طلا (AuNP) برای شناسایی ویروس اس سیب زمینی (Potato virus S, PVS) به کار گرفته شد. AuNP از طریق احیاء سیترات تهیه شد و سپس آنتی­بادی اختصاصی پروتئین پوششی PVS به آ­نها متصل شد. بهینه سازی واکنش اتصال با تغییر اسیدیته AuNPs و غلظت آنتی­بادی انجام گرفت. با استفاده از نانوذرات پوشش داده شده با آنتی­بادی، تشخیص آنتی­ژن در غلظت­های مختلف با طیف­سنجی انجام شد. پس از اتصال آنتی­بادی به نانوذرات طلا، بیشینه جذب نانوذره به علت افزایش اندازه ذرات به مقدار 4-5 نانومتر تغییر کرده و از 524 به 530 نانومتر رسید. اتصال آنتی­ژن به نانوذره نیز موجب تغییر اندازه ذرات و افزایش بیشینه جذب از 530 به 539 نانومتر شد. طیف جذبی نانوذره در  هنگام استفاده از PVY و CMV تغییر نکرد. این مطالعه اولین تلاش برای استفاده از نانوذرات در تشخیص سریع و آسان PVS است.

کلیدواژه‌ها


عنوان مقاله [English]

Rapid detection of Potato virus S using antibody-coated gold nanoparticles

نویسندگان [English]

  • Nahid Masoudi 1
  • Mohamad Hasan Assareh 2
  • Ahmad Rouhibakhsh 2
  • Rasoul Madani 2
  • Masoud Naderpour 2
  • Tara Emami 2
  • Seyed Mohammad Amini 2
  • Davoud Koolivand 2
1 faculty member
2 faculty member
چکیده [English]

In spite of existing of several methods for detection of viruses, time consuming is major limitation of them. So, developing faster and real time method is important to certificate the plant materials in quarantine stations and virus management. In the recent research, nanotechnology was employed to detect Potato virus S (PVS) using gold nanoparticles (AuNPs). Colloidal AuNPs were prepared through citrate reduction and conjugated to a specific antibody against PVS coat protein. Conjugation was optimized by changing the pH of AuNPs and antibody concentration. Antibody coated nanoparticles were used to detect different concentrations of antigen using spectrophotometry. After binding the antibody to the gold nanoparticles which cause an increase in particle size, the SPR peak was displaced in range of 4-5 nm and shifted from 524 to 530 nm. The binding of the antigen to the nanoparticle also caused a change in the particle size and an increase maximum absorbance from 530 to 539 nm. No spectral change were seen when PVY and CMV used as controls. This study is the first attempt of nanoparticle usage in quick and easy detection of PVS

کلیدواژه‌ها [English]

  • Gold nanoparticles
  • Antibody
  • Biosensors
  • Plasmon Resonance
  • Potato virus S
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