نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان بخش گیاه پزشکی، دانشکده کشاورز ی، دانشگاه جیرفت
2 بخش گیاهپزشکی، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Begomovirus infecting vegetables is one of the main components of yield losses in south-eastern Iran. In this
study, the incidence of chili leaf curl virus (ChiLCV) and the associated satellites were studied in pepper
farms and commercial greenhouses of Jiroft and Manoojan (Kerman province). Full-length genome of three
ChiLCV isolates was amplified using the rolling circle amplification method followed by cloning and
sequencing. Furthermore, associated alpha- and betasatellites genomes were amplified in the PCR assay
using specific and degenerate primer pairs, respectively. Sequence comparison showed that three ChiLCV
isolates shared >95% pairwise nucleotide identities with the selected GenBank isolates. Gossypium darwinii
symptomless alphasatellite (GDarSLA) was also found to be associated with three ChiLCV isolates and
shared >89% pairwise identities with the selected GenBank isolates. Furthermore, tomato leaf curl
betasatellie (ToLCB) was identified in two pepper isolates from Manoojan and shared >94% pairwise
identities with the selected GenBank isolates. To demonstrate the pathogenesis of ChiLCV, a partial dimer of
the viral genome was designed, constructed and agroinoculated into pepper seedlings. This led to the
appearance of typical ChiLCV symptoms, including dwarfing and yellowing. Collectively, ChiLCV is one of
the important begomoviruses infecting pepper in south-eastern Iran and the full-length genome of the virus
as well as the associated alphasatellite are reported for the first time in Iran.
کلیدواژه‌ها [English]
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