نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشجو دکتری بخش گیاهپزشکی، دانشکده کشاورزی دانشگاه شیراز، ایران
2 استاد بخش گیاهپزشکی، دانشکده کشاورزی دانشگاه شیراز، ایران
چکیده
عنوان مقاله [English]
نویسندگان [English]
As a consequence of the abundant use of copper bactericides, some species of plant pathogenic bacteria,
including Xanthomonas citri pv. citri (Xcc), have developed resistance to copper. This study evaluates
pathological phenotypes and in vitro efficiency of prevalent copper compounds in inhibiting Xcc. Sampling
was conducted across citrus orchards during 2021-2022. One hundred and seventeen citrus orchards were
surveyed and 132 strains identified as Xanthomonas citri pv. citri based on phenotypic and genotypic
characteristics. Different pathogenic phenotypes were observed by inoculating the representative strains on
different cultivars of lime, orange, citrumelo and grapefruit, but tangerine cultivars did not show disease
symptoms. All the strains showed an intermediate level of sensitivity and grew in the medium containing 100
and 150 mg/l of copper sulfate. In addition higher concentrations (250, 300, 350 and 400 mg/liter) showed
inhibition rate more than 90%. Copper carboxylate at the concentration of 0.75 g/liter prevented the growth
of more than 85% of the strains. Also, Copper oxychloride showed the best inhibition rate in concentrations
higher than 3 g/liter. 1.5 ml/liter of Bordeaux mixture controlled more than 80% of the strains. None of
copper resistance gene clusters copLAB and copABCD were detected in tested Xanthomonas citri strains. The
proper characterization of pathological phenotypes and sensitivity to copper leads more confident decisions
in citrus canker disease management.
کلیدواژهها [English]
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