نوع مقاله : گزارش کوتاه
نویسندگان
1 بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آمووزش کشواورزی و منوابط بیعو ی اسوتا فوار ، سازما تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایرا ن.
2 بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آمووزش کشواورزی و منوابط بیعو ی اسوتا فوار ، سازما تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایرا .
3 خش تحقیقات گیاهپزشکی، مرکز تحقیقات و آمووزش کشواورزی و منوابط بیعو ی اسوتا فوار ، سازما تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایرا .
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
In a 2022 survey conducted in Jahrom, Fars province, Iran, the dog rose yellows (DRY) disease was
observed in Rosa canina L. plants. The disease manifested as severe yellowing, leaf curling, reduced
flowering, abnormal flowers and stunted growth (Fig. 1). DNA from 15 symptomatic and four asymptomatic
plants were analyzed for phytoplasma presence. Nested PCR with P1/P7 and R16F2n/R16R2 primers (Lee et
al., 1998) yielded 1.25 kbp fragments exclusively from symptomatic plants. The sequences from these
samples were identical and one was submitted to GenBank (Accession No. OQ332406). BLAST analysis
revealed that the Jahrom DRY phytoplasma sequence matched with two Iranian 16SrI-B strains: Bajgah
Tragopogon dubius phyllody (KR262955) and Zarghan onion yellows (MG519854). Phylogenetic analysis
placed DRY phytoplasma within the 16SrI group, closet to the 16SrI-B subgroup (Fig. 2). In Iran, 16SrI-B
phytoplasma subgroup has a wide host range and at least 11 plant species are reported as hosts of this
subgroup (Esmaeilzadeh-Hosseini et al., 2023). Fifteen infected plants relative to 250 plants assessed,
showed a noticeable distribution of the DRY disease. This report marks the first association of a 16SrI-B
phytoplasma with DRY disease in Iran. Circulifer haematoceps leafhopper has been previously reported as
vector of rapeseed phyllody phytoplasma, a 16SrI-B subgroup strain (Salehi et al., 2011) in Iran. However,
the ability of C. haematoceps to transmit this 16SrI-B subgroup phytoplasma to dog rose is yet to be tested.
کلیدواژه‌ها [English]
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