نوع مقاله : مقاله کامل پژوهشی
نویسنده
دانشگاه صنعتی اصفهان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسنده [English]
Abstract
Candidaus Phytoplasma solani and Ca. Phytoplasma trifolii are the dominant causal agents of potato purple top and stolbur diseases in Isfahan province. While leafhoppers are known as the primary vectors of phytoplasmas, the exact impact of seed tubers on the transmission of phytoplasmas across different generations is still uncertain. In this study, 111 potato tubers from 47 phytoplasma-associated plants were grown in an insect-proof greenhouse The nested-PCR assays revealed that 25 second-generation potato plants tested positive for Ca. P. solani (22%), 19 of which were symptomatic. Ca. P. trifolii was not detected in any of these plants. As the third generation, 82 progeny tubers were replanted from the 47 samples. Among these, only five plants were identified as infected with Ca. P. solani (6%), while they were negative for Ca.P. trifolii. The elevated titer of Ca. P. solani within the host may have played a significant role in enhancing the seed transmission of the pathogen to subsequent generations. The decrease in the transmission rate of Ca. P. solani in offspring suggests that the uneven distribution of phytoplasma within the phloem may result in circumstances where not all tuber derived from an infected maternal tuber becomes infected. Despite a relatively low-rate of phytoplasma transmission across successive generations of seed potatoes, this reduced transmission level remains significant as a source of phytoplasma inoculum, facilitating the spread of the disease through leafhopper vectors.
کلیدواژهها [English]
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