نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 گروه گیاهپزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری/
2 پژوهشکده بیوتکنولوژی صنعتی ، جهاددانشگاهی خراسان رضوی
3 گیاه پزشکی، دانشکده کشاورزی دانشگاه زابل
4 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل
5 گروه گیاهپزشکی، دانکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Bacterial blight caused by Xanthomonas oryzae pv. oryzae is one of the most destructive rice diseases in most regions. Understanding the biochemical and molecular interaction plays an important role in designing a specific strategy for the improvement and resistance of plants as the most effective and economical management method. In the interaction between the pathogen and the host, hundreds of genes are regulated and expressed, and in most cases, the difference between resistance and sensitivity is related to time and the amount of these changes compared to the difference in the expression of a set of genes. This study evaluated the expression pattern of NH1, thionine, and lipoxygenase genes at different intervals after inoculation in resistant (Khazar) and susceptible (local Tarom) cultivars to bacterial blight using qRT-PCR. The study of the expression pattern of the investigated genes showed that the trend of increasing their expression in the first period after inoculation (12 hours) in the resistant variety Khazar was significantly different from the susceptible variety. Despite the decrease in the expression of these genes after the initial hours, the gene expression ratio in the two susceptible and resistant varieties was significantly different at the 5% level up to 72 hours after inoculation. Increasing the expression of the mentioned genes and inducing various related systemic resistance pathways as a part of the defense mechanism of rice has played an important role in the resistance of the commercial cultivar Khazar to rice bacterial blight.
کلیدواژه‌ها [English]
)