نوع مقاله : گزارش کوتاه
نویسندگان
1 دانشجوی دکتری بیماری شناسی گیاهی دانشگاه شیراز
2 بخش گیاهپزشکی، مرکز تحقیقات ویروس شناسی گیاهی، دانشکده کشاورزی، دانشگاه شیراز
3 2- استادیار بیماری شناسیگیاهی، دانشگاه پیام نور، تهران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Emerging plant viral diseases account for serious threats to agricultural crop yields and food security in many parts of the world. in spring 2023 revealedsevere leaf chlorotic spots, yellowing symptoms, and fruit necrosis in tomato (Haji-Abad) and pepper (Bandar-Abbas) plants (Figure 1). To confirm AMV infections, total RNA was extracted from five symptomatic and one asymptomatic samples of tomato and pepper plants using TRIzol reagent (Sinaclone), followed by reverse transcription-polymerase chain reaction (RT-PCR) assays with a set of specific AMV primers (AMV-R, AMV-F) targeting a 780-nucleotide segment of the coat protein gene (Masoumi et al., 2012). PCR products of the expected size (approximately 780 bp) were obtained from all of the symptomatic samples (five samples of tomato and pepper plants), while no amplified products were generated from any of the healthy leaf samples of tomato and pepper plants. and sequenced directly using a paired-end sequencing strategy (Sinohe Co. Iran). Multiple alignments of the obtained nucleotide sequences (corresponding to a part of CP gene) with 15 AMV isolates obtained from GenBank showed the highest identity (97.9%. for IR-To-01 isolate and 98.3% for IR-Pep-03 isolate) with an Italian AMV strain See-1 isolated from Sechium elude and the lowest identity (95.8% for IR-To-01 and 95.4% for IR-Pep-03) with an Iranian AMV isolate (IRWS2) from Wisteria sinensis. Phylogenetic analysis grouped Iranian isolates with European strains, while, the other AMV isolates (including Iranian, Asian and American isolates) were grouped into a separate clade (Figure 2), suggesting a possible introduction through seed trade.
کلیدواژهها [English]
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