نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان
2 دانشگاه شهید باهنر کرمان
3 Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Faba bean necrotic yellows virus (FBNYV; Nanovirus necroflaviviciae, Nanoviridae) is one of the most important viruses infecting leguminous plants in Asia, Africa and Europe. In the current research, eight genomic components of two chickpea and lentil isolates of the virus, as well as six genomic components of the fenugreek isolate, collected from farms in Northern Khorasan and Razavi Khorasan Provinces, respectively, were amplified using polymerase chain reaction, cloned and sequenced. Comparison of the sequences of concatenated genome components of DNA-R, DNA-S, DNA-M, DNA-N, DNA-U1 and DNA-U4 of the three isolates showed that they shared nucleotide identity of 94.9%-96.20%. Among the six components, DNA-R and DNA-M were found to be the most and the least diverse components relative to the others (99.1% and 99.3% and 85.9%-97.8% identities, respectively). Analysis of the constructed phylogenetic tree showed that three studied isolates were clustered with other Iranian, as well as two Azerbaijani GenBank isolates, in a district group. Furthermore, the pattern of the geographic distribution of all Iranian FBNYV isolates is related to their phylogeny. These results affirm that in addition to leguminous plants, FBNYV is prevalent in leafy vegetable farms in Iran.
کلیدواژهها [English]
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