نوع مقاله : گزارش کوتاه
نویسندگان
دانشگاه شهید باهنر کرمان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Geminiviruses are common viruses in vegetable and crop fields in Kerman Province. In this research, to identify begomoviruses infecting plants in the farms of Kerman Province, three symptomatic okra samples (Abelmoschus esculentus L., Malvaceae) showing blistering, vein swelling, leaf crinkling, and malformation symptoms (Figs. 1a and 1b) were collected from research farm in Agricultural college, Shahid Bahonar University of Kerman. Total DNA of samples were extracted and subjected to PCR using specific primers of common begomoviruses and the associated betasatellite in this region including okra enation leaf curl virus (OELCuV; Begomovirus abelsmoschusenation), cotton leaf curl Gezira virus (CLCuGeV; B. gossypigeziraense), watermelon chlorotic stunt virus (WmCSV; B. citrulli), tomato leaf curl Palampur virus (ToLCPalV; B. solanumpalampurense) and okra leaf curl Oman betasatellite (OLCuOMB, Betasatellite abelmoschusomanense) (Esmaeili et al. 2016; Heydari et al. 2025; Heydarnejad et al. 2013; Salari et al. 2023) (Table 1). Based on the results of PCR assays, fragments of expected sizes from OELCuV, CLCuGeV and OLCuOMB genomes, as well as DNA-A and DNA-B components from WmCSV and ToLCPalV genomes were amplified for all three samples (Fig. 2; only PCR results of two samples are shown in the gel).
کلیدواژهها [English]
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