نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 عضو هیات علمی دانشگاه بیرجند
2 دانشجوی دکترا
3 محقق مرکز تحقیقات کشاورزی سیراد- کشور فرانسه
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Saffron (Crocus sativus L.) is one of Iran’s most valuable crops, both economically and culturally. It is vegetatively propagated through corms and is affected by viral infections that reduce its yield and quality. In this study, to investigate the viral infection status of saffron and to molecularly identify Saffron yellow mosaic virus (SYMV), 51 saffron leaf samples were collected from fields in South and Razavi Khorasan provinces in 2024. Virion-associated nucleic acid (VANA) extraction was performed, followed by sequencing using 454 pyrosequencing technology. Metagenomic analysis revealed that viruses belonging to the genus Potyvirus, particularly SYMV, were the most prevalent in Iranian saffron populations. RT-PCR assays using Potyvirus-specific primers confirmed the presence of SYMV in four regions: Qaen, Ferdows, Birjand, and Torbat Heydariyeh. The complete genome sequence of the Iranian isolate (Ferdows-SYMV.IR) was determined to be 8,917 nucleotides long, and a phylogenetic tree was constructed using the Maximum Likelihood method. Phylogenetic analysis showed that Iranian SYMV isolates shared 97–100% nucleotide identity with the reference isolate (OK632024) and were distinct from closely related viruses such as Bean yellow mosaic virus and Clitoria yellow vein virus. Observed symptoms in infected plants included yellowing, mosaic patterns, and leaf curling. This study provides the first definitive evidence of SYMV presence in South Khorasan province and its detection in Razavi Khorasan. The findings highlight the importance of continuous monitoring, development of specific diagnostic tools, and vector management strategies to prevent the spread of emerging saffron viruses.
کلیدواژهها [English]
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