نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 نویسنده
2 مسئول مکاتبه
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Wheat rusts including stripe and stem rusts are the most important fungal diseases in the world and Iran as frequently causes natural epidemics and significant yield loses due to rapid development of new races in life cycle resulted from mutation or sexual recombination. In this research, distribution and genetic diversity of aecial infection on barberry bushes and their relation to wheat yellow rust was studied. A total of 95 samples of infected leaves to aecial stage on barberry bushes were collected from Borujerd, Dorud, Azna and Aligudarz regions. To identify the rust species, in vivo and in vitro pathogenicity test were conducted by artificial inoculation of wheat seedlings using collected aeciospores from each region.The results showed the production of urediniospores of P. striiformis f. sp. tritici (yellow rust) as well as P. graminis f. sp. tritici (black rust) in inoculated wheat seedlings. Molecular detection and sequencing of the rDNA regions of representative aecial isolates on Barberry were done. Genetic diversity of 134 aecial isolates was also studied by amplification of IGS1-rDNA region. The Results also confirmed the pathogenicity test and showed that barberry could be the aecial host of yellow and black rusts in Lorestan province. The IGS-rDNA amplification of aecial isolates from Azna and Aligudarz showed most a production of three bands with a frequency of 15 and 21 isolates respectively. In Dorud samples was with five bands (26 isolates) as well as in Broujerd samples with two bands (10 isolates) in which showed a genetic variation among aecial isolates collected from sampling regions in Lorestan province.
کلیدواژهها [English]
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