ارزیابی فنوتیپی و مولکولی مقاومت به بیماری زنگ سیاه در برخی از ارقام تجاری و لاین-های امیدبخش گندم

نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 استادیار پژوهش، سازمان تحقیقات اموزش کشاورزی و منابع طبیعی

2 استادیار پژوهش، موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

3 دانشکده کشاورزی دانشگاه تبریز

4 موسسه تحقیقات اصلاح و تهیه نهال و بذر

چکیده

بیماری‌های قارچی از عوامل عمده‌ی کاهش محصول و افزایش هزینه‌های تولید گندم به‌شمار می‌روند. زنگ سیاه با عامل (Pgt) Puccinia graminis f. sp. tritici، یکی از مهم‌ترین بیماری‌های قارچی گندم در جهان می‌باشد. شناسایی منابع مقاومت جدید به زنگ‌ سیاه و تعیین ژن‌های مقاومت موجود در آن‌ها، از ملزومات تولید ارقام مقاوم و ایجاد مقاومت پایدار به‌عنوان مهم‌ترین روش کنترل این بیماری‌ محسوب می‌شود. به‌منظور شناسایی منابع جدید مقاومت گیاهچه‌ای نسبت به بیماری زنگ سیاه، الگوی بیماری‌زایی هشت جدایه‌ی مختلف Pgt بر روی 27 رقم تجاری و لاین امیدبخش گندم حاصل از برنامه‌های ملی به‌نژادی کشور در قالب طرح بلوک‌های کامل تصادفی با سه تکرار بررسی شد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Phenotypic and Molecular Resistance to Stem Rust Disease in Wheat Commercial Cultivars and Elite Lines

نویسندگان [English]

  • Ali Omrani 1
  • Ramin roohparvar 2
  • Saeed Aharizad 3
  • Manoochehr khodarahmi 4
  • Mahmoud Toorchi 3
1 Agricultural Research, Education and Extension Organization (AREEO),
2 Assist. Prof., Seed and Plant Improvement Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
3 Faculty of Agriculture, University of Tabriz
4 Seed and Plant Improvement Institute
چکیده [English]

Fungal diseases are considered as main causes of yield loss and increased costs in wheat production. Stem rust caused by Puccinia graminis f. sp. tritici (Pgt), is a devastating fungal disease of wheat, worldwide. Identification of new stem rust resistance sources, and the involved genes are required to achieve resistant cultivars and durable resistance as the most efficient control method of wheat stem rust. In order to identify new seedling resistance sources, virulence pattern of eight Pgt isolates was studied on 27 wheat commercial cultivars and elite lines of national breeding programs in a randomized block design with three replications.

کلیدواژه‌ها [English]

  • Black rust
  • Yield loss
  • Resistance sources
  • Puccinia graminis

مراجع

Afshari, F., Aghaee, M., Jalal Kamali, M.R., Roohparvar, R., Malihipour, A., Khodarahmi, M., Ebrahimnejad, Sh., Aghnum, R., Chaichi, M., Dadrezaei, S.T., Dalvand, M., Dehghan, M.A., Zakeri, A.K., Shahbazi, K., Safari, S.A., Tabatabaei, N., Atahoseini, M., Nabati, E., Hooshyar, R., Yasaei, M., Nasrollahi, M., Mehrabi, R., Ghaffary, T., Hashemi, M., Patpour, M., Bayat, Z. 2015. Surveillance and Pgt race analysis in Iran, 2014. Borlaug Global Rust Initiative, 123pp.
Anonymous. 2021. MAS Wheat. Available at https://maswheat.ucdavis.edu/protocols/Sr36/.
Bariana, H.S. and McIntosh, R.A. 1994. Characterisation and origin of rust and powdery mildew resistance genes in VPM1 wheat. Euphytica, 76(1): 53-61.
Bhattacharya S. 2017. Deadly new wheat disease threatens Europe's crops. Nature, 542:145-146.
Chen, S., Rouse, M. N., Zhang, W., Jin, Y., Akhunov, E., Wei, Y., & Dubcovsky, J. 2015. Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group. Theoretical and Applied Genetics, 128(4): 645-656.
Dadrezaei, S.T. and Nazari, K. 2015. Detection of wheat rust resistance genes in some Iranian wheat genotypes by molecular markers. Seed and Plant Improvement Journal, 31(3): 163-187 (in Persian).
Ejaz, M., Iqbal, M., Shahzad, A., Ahmed, I., and Ali, G. M. 2012. Genetic variation for markers linked to stem rust resistance genes in Pakistani wheat varieties. Crop Science, 52(6): 2638-2648.‏
Francia, E., Tacconi, G., Crosatti, C., Barabaschi, D., Bulgarelli, D., Dall’Aglio, E., and Valè, G. 2005. Marker assisted selection in crop plants. Plant Cell, Tissue and Organ Culture, 82(1): 317-342.
Jin, Y., Szabo, L. J., Pretorius, Z. A., Singh, R. P., Ward, R., and Fetch, T. 2008. Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Disease 92(1): 923-926.
Li, T.Y., Cao, Y.Y., Wu, X.X., Xu, X.F. and Wang, W.L. 2016. Seedling resistance to stem rust and molecular marker analysis of resistance genes in wheat cultivars of Yunnan, China. Plos One, 11(10): 0165640.
Liu, S., Yu, L.X., Singh, R.P., Jin, Y., Sorrells, M.E. and Anderson, J.A. 2010. Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26. Theoretical and Applied Genetics, 120(4): 691-697.
Mago, R., Bariana, H.S., Dundas, I.S., Spielmeyer, W., Lawrence, G.J., Pryor, A.J. and Ellis, J.G. 2005. Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theoretical and Applied Genetics, 111(3): 496-504.
McCallum, B.D., Hiebert, C.W., Cloutier, S., Bakkeren, G., Rosa, S.B., Humphreys, D.G., Marais, G.F., McCartney, C.A., Panwar, V., Rampitsch, C. and Saville, B.J. 2016. A review of wheat leaf rust research and the development of resistant cultivars in Canada. Canadian Journal of Plant Pathology, 38(1): 1-18.
McIntosh, R.A., Wellings, C.R. and Park, R.F. 1995. Wheat Rusts: An Atlas of Resistance Genes. Australia: CSIRO. Publications, Victoria, Australia. 200 pp.
Nazari, K., Mafi, M., Yahyaoui, A., Singh, R. P., and Park, R. P. 2009. Detection of wheat stem rust (P. graminis f. sp. tritici) race TTKSK (Ug99) in Iran. Plant Disease, 93:317.
Olivera P, Newcomb M, Szabo LJ, Rouse M, Johnson J, Gale S, Luster DG, Hodson D, Cox JA, Burgin L, Hort M, Gilligan CA, Patpour M, Justesen AF, Hovmøller MS, Woldeab G, Hailu E, Hundie B, Tadesse K, Pumphrey M, Singh RP, Jin Y. 2015. Phenotypic and genotypic characterization of race TKTTF of Puccinia graminis f. sp. tritici that caused a wheat stem rust Epidemic in Southern Ethiopia in 2013-14. Phytopathology, 105(2):917-928.
Omrani, A., Aharizad, S., Roohparvar, R., Khodarahmi, M., and Toorchi, M. 2017. Identification of stem and leaf rust resistance genes in some promising wheat lines using molecular markers. Journal of Crop Biotechnology, 18(3): 15-25 (in Persian).
Omrani, A. and Roohparvar, R. 2020. First report of TTKTK, a variant of the race TTKSK (Ug99) of Puccinia graminis f. sp. tritici with virulence on the resistance genes Sr31 and SrTmp in Iran. Journal of Applied Research in Plant Protection 9(3): 87–89. (In Persian)
Omrani, A. and Roohparvar, R. 2021a. First report of TTRTF race of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici from Iran (Northwest, Cold Zone). Journal of Applied Research in Plant Protection 9(4): 101–103. (In Persian)
Omrani, A, Roohparvar, R. 2021b. Occurence of the TTKSK (Ug99) race of Puccinia graminis f. sp. tritici from northwest of Iran (Hashtrood region). Journal of Applied Research in Plant Protection 10 (2): 91–93. (In Persian)
Patpour, M. 2013. Study on genetic and virulence diversity of Puccinia graminis f. sp. tritici populations in Iran and stem rust resistance genes in wheat. Ph.D. Thesis in agricultural biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran, 165 pp.
Patpour, M., Justesen, A.F., Tecle, A.W., Yazdani, M. and Hovmøller, M.S. 2020. First report of race TTRTF of wheat stem rust (Puccinia graminis f. sp. tritici) in Eritrea. Plant Disease.
Patpour, M., Nazari, K., Ogbonnaya, F., Alavi, S.M. and Mousavi, A. 2014. Phenotypic and molecular characterization of resistance to stem rust in wheat cultivars and advanced breeding lines from Iran and Syria. Crop Breeding Journal, 4(1):1-14.
Roelfs, A.P., Singh, R.P. and Saari, E.E. 1992. Rust Disease of Wheat: Concepts and Methods of Disease Management. CIMMIT, Mexico, D.F. 81 pp.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A. and Allard, R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences, 81(1): 8014-8019.
Singh, R.P., Hodson, D.P., Huerta-Espino, J., Jin, Y., Njau, P., Wanyera, R., Herrera-Foessel, S.A. and Ward, R.W., 2008. Will stem rust destroy the world's wheat crop? Advances in agronomy, 98(2): 271-309.
Singh, R.P., Hodson, D.P., Jin, Y., Lagudah, E.S., Ayliffe, M.A., Bhavani, S., Rouse, M.N., Pretorius, Z.A., Szabo, L.J., Huerta-Espino, J., Basnet, B.R., Lan, C., and Hovmøller, M.S. 2015. Emergence and spread of new races of wheat stem rust fungus: Continued threat to food security and prospects of genetic control. Phytopathology, 105(3): 872-884.
Stakman, E.C., Stewart, D.M. and Loegering, W.Q. 1962. Identification of physiologic races of P. graminis var. tritici. USDA, Agricultural Research Service. E617, Washington, USA.
Tsilo, T.J., Jin, Y. and Anderson, J.A. 2008. Diagnostic microsatellite markers for the detection of stem rust resistance gene in diverse genetic backgrounds of wheat. Crop Science, 48(1): 253-261.
Xu, X.F., Li, D.D., Liu, Y., Gao, Y., Wang, Z.Y., Ma, Y.C., Yang, S., Cao, Y.Y., Xuan, Y.H., and Li, T.Y. 2017. Evaluation and identification of stem rust resistance genes Sr2, Sr24, Sr25, Sr26, Sr31 and Sr38 in wheat lines from Gansu Province in China. PeerJ, 5, e4146. ‏
Yang, H., Li, C., Lam, H.M., Clements, J., Yan, G. and Zhao, S. 2015. Sequencing consolidates molecular markers with plant breeding practice. Theoretical Applied Genetics, 128(1): 779-795.
Yu, L.X., Liu, S., Anderson, J.A., Singh, R.P., Jin, Y., Dubcovsky, J., Brown-Guidera, G., Bhavani, S., Morgounov, A., He, Z. and Huerta-Espino, J. 2010. Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines. Molecular breeding, 26(4): 667-680.
Yu, L.X., Liu, S., Anderson, J.A., Singh, R.P., Jin, Y., Dubcovsky, J., Brown-Guidera, G., Bhavani, S., Morgounov, A., He, Z. and Huerta-Espino, J. 2012. Erratum to: Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines. Molecular Breeding, 30(1): 613-614.
بیماریهای گیاهی
دوره 57، شماره 2
شهریور 1400
صفحه 109-126

فایل ها

هم رسانی

ارجاع به این مقاله

آمار